Proteomic study of sera from patients with bladder cancer: usefulness of S100A8 and S100A9 proteins.

Abstract

To effectively obtain tumour-specific markers, fractionated proteins obtained using reversed-phase high-performance liquid chromatography for patient-matched pre- and postoperative sera from bladder cancer patients were compared by two-dimensional gel electrophoresis. The usefulness of the identified proteins was confirmed immunohistochemically. S100A8 and S100A9 were identified as tumour-associated proteins. The increased immunoreactive expression of S100A8 protein was associated with bladder wall muscle invasion of the tumour and cancer-specific survival (p<0.05), and the increased immunoreactive expression of S100A9 protein was associated with the tumour grade (p<0.05). In addition, increased expressions of both proteins was associated with recurrence-free survival at a median follow-up of 32.9 months (both p<0.05). On multivariate analysis, the expression of S100A8 was a significant predictor of recurrence (p<0.05). These findings may help to identify biologically aggressive tumors and, thus, patients who might benefit from more intensive adjuvant therapy.

• Authors: Sho Minami, Yuichi Sato, Toshihide Matsumoto, Taihei Kageyama, Yusuke Kawashima, Kodera Yoshio, Jun-Ichiro Ishii, Kazumasa Matsumoto, Ryo Nagashio, Isao Okayasu
• Journal: Cancer genomics & proteomics (Cancer Genomics Proteomics) 2010 Jul-Aug Vol. 7 Issue 4 Pg. 181-9 ISSN: 1790-6245 [Electronic] Greece
• PMID: 20656984 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Calgranulin A
• Calgranulin B

Topics

• Calgranulin A (blood)
• Calgranulin B (blood)
• Female
• Humans
• Male
• Prognosis
• Proteomics
• Reproducibility of Results
• Urinary Bladder Neoplasms (blood, diagnosis)