Abstract | BACKGROUND: OBJECTIVE: METHOD: In this investigation, 50 children with Guillain-Barre syndrome (GBS) who were admitted from July 2006 to July 2008, to Tabriz Children's Hospital in the northwest of Iran were studied. 30 children admitted for various other reasons than GBS were randomly selected as a control group. The levels of anti- ganglioside antibodies in serum were measured by ELISA and immunoblotting methods using commercial kits. RESULTS: Anti- ganglioside antibodies ( IgG) were detected in 16 (32%) GBS patients and in 1 (3.3%) control using ELISA assay. However, by employing immunoblotting technique, antibodies against seven gangliosides were found positive in 28 (56%) of GBS patients and none in the control group. The sensitivities of immunoblotting and ELISA methods were 56% and 32% and their specificities were 100% and 97%, respectively (p<0.001). CONCLUSION: According to the clinical criteria of GBS, the specificity and sensitivity of immunoblotting was better than those of ELISA. It is important to notice that the immunoblotting method is able to measure the seven types of antibodies (GM1, GM2, GM3, GD1a, GD1b, GT1b, and GQ1b) simultaneously and it is an easy, routine method with a lower cost.
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Authors | Mohammad Reza Bonyadi, Mohammad Barzegar, Reza Badalzadeh, Mazyar Hashemilar |
Journal | Iranian journal of immunology : IJI
(Iran J Immunol)
Vol. 7
Issue 2
Pg. 117-23
(Jun 2010)
ISSN: 1735-1383 [Print] Iran |
PMID | 20574125
(Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Autoantibodies
- Gangliosides
- Immunoglobulin Isotypes
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Topics |
- Autoantibodies
(blood, immunology)
- Child
- Child, Preschool
- Cost-Benefit Analysis
- Enzyme-Linked Immunosorbent Assay
(economics, methods)
- Female
- Gangliosides
(immunology)
- Guillain-Barre Syndrome
(blood, diagnosis, immunology)
- Humans
- Immunoblotting
(economics, methods)
- Immunoglobulin Isotypes
- Infant
- Male
- Sensitivity and Specificity
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