Gamma' fibrinogen: evaluation of a new assay for study of associations with cardiovascular disease.

Abstract

BACKGROUND:

Studies of disease associations with gamma' fibrinogen, a newly emerging risk factor for cardiovascular disease, have been hampered by the lack of a standardized and well-characterized assay.

METHODS:

We developed an immunometric technique to measure gamma' fibrinogen concentrations in plasma and studied the clinical utility of this test in samples from healthy individuals enrolled in the Framingham Offspring Study and in a separate case/control study of coronary artery disease (CAD). Monoclonal antibody 2.G2.H9, specific for the unique carboxyl terminal peptide of the fibrinogen gamma' chain, was used as capture antibody. Sheep antihuman fibrinogen/horseradish peroxidase conjugate was used for detection, with 3,3',5,5'-tetramethylbenzidine as substrate. We evaluated the linearity, imprecision, analytical specificity, and lower limit of quantification of the assay. We determined the reference interval for gamma' fibrinogen in healthy individuals from the Framingham Offspring Study (n = 2879) and quantified associations between gamma' fibrinogen and cardiovascular disease risk factors. The sensitivity and specificity of gamma' fibrinogen in evaluating CAD patients (n = 133) was determined with ROC curve analysis.

RESULTS:

The gamma' fibrinogen ELISA had within-run CVs of 13.4% at 0.127 g/L and 4.8% at 0.416 g/L. The limit of quantification at an imprecision of 20% was 0.10 g/L. The reference interval for healthy individuals was 0.088-0.551 g/L. ROC curve analysis of results from patients with CAD yielded an area under the curve of 0.76, with a diagnostic accuracy of 0.78 at a decision threshold of 0.30 g/L.

CONCLUSIONS:

gamma' Fibrinogen shows excellent utility for cardiovascular risk analysis.

• Authors: Rehana S Lovely, Steven C Kazmierczak, Joseph M Massaro, Ralph B D'Agostino Sr, Christopher J O'Donnell, David H Farrell
• Journal: Clinical chemistry (Clin Chem) Vol. 56 Issue 5 Pg. 781-8 (May 2010) ISSN: 1530-8561 [Electronic] England
• PMID: 20348406 (Publication Type: Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Validation Study)

Chemical References

• Antibodies, Monoclonal
• Fibrinogen

Topics

• Adult
• Aged
• Aged, 80 and over
• Antibodies, Monoclonal (immunology)
• Cardiovascular Diseases (blood, diagnosis)
• Coronary Artery Disease (blood, diagnosis)
• Enzyme-Linked Immunosorbent Assay (methods)
• Female
• Fibrinogen (analysis, immunology)
• Humans
• Limit of Detection
• Male
• Middle Aged
• ROC Curve
• Risk Factors