Betulinic acid a radiosensitizer in head and neck squamous cell carcinoma cell lines.

Abstract

BACKGROUND AND PURPOSE:

Betulinic acid, a pentacyclic triterpene, is a new cytotoxic compound active on melanoma, neuroblastoma, glioblastoma and head and neck squamous cell carcinoma (HNSCC) cells. In combination with irradiation it has been shown to have an additive effect on growth inhibition in melanoma cells. In this study, the radiosensitizing effect of betulinic acid on sequential irradiation was investigated in HNSCC cell lines.

MATERIAL AND METHODS:

Two HNSCC cell lines, SCC9 and SCC25, were treated with increasing doses of betulinic acid and sequentially irradiated with a single boost of 4 Gy from a conventional radiation source. The cells were counted, the surviving fraction was determined, and colony-forming assays were performed.

RESULTS:

It could be shown that betulinic acid alone inhibits cell survival, affects cell survival additively in combination with irradiation and decreases clonogenic survival in both cell lines when applied alone.

CONCLUSION:

Betulinic acid could be a promising treatment agent in radioresistant head and neck cancer. A combination of betulinic acid with radiotherapy seems to be beneficial.

• Authors: Christina Eder-Czembirek, Boban M Erovic, Cornelia Czembirek, Markus Brunner, Edgar Selzer, Richard Pötter, Dietmar Thurnher
• Journal: Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [et al] (Strahlenther Onkol) Vol. 186 Issue 3 Pg. 143-8 (Mar 2010) ISSN: 1439-099X [Electronic] Germany
• PMID: 20339825 (Publication Type: Journal Article)

Chemical References

• Pentacyclic Triterpenes
• Radiation-Sensitizing Agents
• Triterpenes
• Betulinic Acid

Topics

• Carcinoma, Squamous Cell (pathology, radiotherapy)
• Cell Line
• Cell Survival
• Dose-Response Relationship, Drug
• Head and Neck Neoplasms (pathology, radiotherapy)
• Humans
• Pentacyclic Triterpenes
• Radiation-Sensitizing Agents
• Radiotherapy Dosage
• Treatment Outcome
• Triterpenes (administration & dosage)
• Betulinic Acid