Abstract | BACKGROUND: METHODS: Three heparanase-specific small interfering RNA (siRNAs) were designed, synthesized, and transfected into cultured gastric cancer cell line SGC-7901. Heparanase expression was measured by RT-PCR, real-time quantitative PCR and Western blot. Cell proliferation was detected by MTT colorimetry and colony formation assay. The in vitro invasion and metastasis of cancer cells were measured by cell adhesion assay, scratch assay and matrigel invasion assay. The angiogenesis capabilities of cancer cells were measured by tube formation of endothelial cells. RESULTS: Transfection of siRNA against 1496-1514 bp of encoding regions resulted in reduced expression of heparanase, which started at 24 hrs and lasted for 120 hrs post-transfection. The siRNA-mediated silencing of heparanase suppressed the cellular proliferation of SGC-7901 cells. In addition, the in vitro invasion and metastasis of cancer cells were attenuated after knock-down of heparanase. Moreover, transfection of heparanase-specific siRNA attenuated the in vitro angiogenesis of cancer cells in a dose-dependent manner. CONCLUSIONS:
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Authors | Liduan Zheng, Guosong Jiang, Hong Mei, Jiarui Pu, Jihua Dong, Xiaohua Hou, Qiangsong Tong |
Journal | BMC cancer
(BMC Cancer)
Vol. 10
Pg. 33
(Feb 05 2010)
ISSN: 1471-2407 [Electronic] England |
PMID | 20137078
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- RNA, Small Interfering
- heparanase
- Glucuronidase
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Topics |
- Cell Adhesion
(physiology)
- Cell Growth Processes
(physiology)
- Cell Line, Tumor
- Cell Movement
(physiology)
- Gene Silencing
- Glucuronidase
(antagonists & inhibitors, biosynthesis, genetics)
- Humans
- Neoplasm Invasiveness
- Neoplasm Metastasis
- Neovascularization, Pathologic
(enzymology, genetics, pathology)
- RNA, Small Interfering
(administration & dosage, genetics)
- Stomach Neoplasms
(blood supply, enzymology, genetics, pathology)
- Transfection
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