[The relationship among IL-10, TNF gene polymorphisms, Helicobacter pylori infection and gastroduodenal diseases in Hubei Han ethnic].

Abstract

OBJECTIVE:

To study the distribution of IL-10 and TNF gene polymorphisms in patients with gastroduodenal diseases in Hubei Han ethnic and their association with Helicobacter pylori (Hp) infection.

METHODS:

Six hundred and five patients with gastroduodenal diseases (196 chronic gastritis, 189 gastroduodenal ulcer and 220 gastric cancer) as well as 624 healthy controls were genotyped with PCR-RFLP method for IL-10-1082,-819,-592 and TNFalpha-308, lymphotoxin-alpha (LTalpha) Nco I and AspH I gene polymorphisms. Hp infection status was determined with ELISA.

RESULTS:

(1) There was significant difference of IL-10-1082 AG + GG genotype among the gastric cancer group with the non-malignant gastric diseases groups and healthy control group (P < 0.05). There was no significant difference of IL-10-592 and -819 gene polymorphisms among gastric cancer patients, non-malignant gastric disease patients and healthy controls (P > 0.05). The genotype frequencies of IL-10-819 were the same as those of IL-10-592. (2) Frequency of IL-10-1082 AG + GG genotype in gastric cancer patients with positive Hp was significantly higher than that in the other three groups (P < 0.05). (3) Frequency of LTalpha Nco I AG genotype in gastric cancer patients with Hp infection was significantly higher than that in Hp positive healthy controls (P < 0.05). There were no other associations between TNFalpha-308, LTalpha Nco I and AspH I gene polymorphisms and Hp infection in gastroduodenal diseases.

CONCLUSIONS:

(1) Allele AG + GG of IL-10-1082 was associated with gastric cancer in Han nationality of Hubei province. (2) IL-10-1082 AG + GG, LTalpha Nco I AG heterozygous genotype may be associated with Hp infection in patients with gastric cancer in Han nationality of Hubei province.

• Authors: Hui Xiao, Chun Li, Yi Jiang, Rui Li, Bing Xia
• Journal: Zhonghua nei ke za zhi (Zhonghua Nei Ke Za Zhi) Vol. 48 Issue 7 Pg. 552-6 (Jul 2009) ISSN: 0578-1426 [Print] China
• PMID: 19957794 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Lymphotoxin-alpha
• Tumor Necrosis Factor-alpha
• Interleukin-10

Topics

• Adult
• Aged
• Duodenal Diseases (ethnology, genetics, microbiology)
• Female
• Gene Frequency
• Genotype
• Helicobacter Infections (ethnology, genetics)
• Humans
• Interleukin-10 (genetics)
• Lymphotoxin-alpha (genetics)
• Male
• Middle Aged
• Polymorphism, Genetic
• Stomach Diseases (ethnology, genetics, microbiology)
• Tumor Necrosis Factor-alpha (genetics)