[Effects of Annexin II gene silencing by siRNA on proliferation and invasive potential of Jurkat lymphoma cells].

Abstract

OBJECTIVE:

To investigate the effects of Annexin II (AnxA2) gene silencing by siRNA on proliferation and invasive potential of lymphoma cell line Jurkat cells.

METHODS:

A synthesized siRNA duplex targeting to AnxA2 was transfected into Jurkat cells. Transfection efficiency was analyzed by real-time PCR and flow cytometry. MTT assay for cell proliferation and transwell plates for invasive potential were performed.

RESULTS:

Compared with the negative controls, the cell proliferation inhibitory rate of the AnxA2 siRNA transfected Jurkat cells was significantly increased at 24 h, 48 h and 72 h [(17.4 +/- 2.3)%, (22.4 +/- 3.8)%, (37.6 +/- 1.5)% vs (-1.3 +/- 5.1)%, (-5.5 +/- 4.4)%, (-10.8 +/- 5.5)%, respectively, P<0.05]. The cell invasive potential of the transfected Jurkat cells was inhibited remarkably at 48 h (11.3 +/- 4.2 vs 54.3 +/- 8.7, P<0.01).

CONCLUSION:

AnxA2 gene silenced by siRNA can inhibit the proliferation and the invasive potential of Jurkat cells remarkably.

• Authors: Hong-yu Bao, Miao Jiang, Zhen-ni Ma, Fei Sheng, Ming-qing Zhu, Lin Chen, Li-qian Xie, Ning-zheng Dong, Chang-geng Ruan
• Journal: Zhonghua xue ye xue za zhi = Zhonghua xueyexue zazhi (Zhonghua Xue Ye Xue Za Zhi) Vol. 30 Issue 5 Pg. 303-6 (May 2009) ISSN: 0253-2727 [Print] China
• PMID: 19799124 (Publication Type: English Abstract, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Annexin A2
• RNA, Small Interfering

Topics

• Annexin A2 (genetics, metabolism)
• Cell Proliferation
• Chemotaxis (genetics)
• Gene Silencing
• Humans
• Jurkat Cells
• RNA, Small Interfering (genetics)
• Transfection