Abstract | HYPOTHESIS: Lyophilized plasma (LP) is as safe and effective as fresh frozen plasma (FFP) for resuscitation after severe trauma. DESIGN: Multicenter animal study. SETTING: Animal laboratories, 2 level I trauma centers. PARTICIPANTS: Thirty-two Yorkshire crossbred swine. INTERVENTIONS: Lyophilized plasma was analyzed for factor levels and clotting activity before lyophilization and after reconstitution. Swine were subjected to complex multiple trauma including extremity fracture, hemorrhage, severe liver injury, acidosis, and hypothermia. They were then resuscitated with FFP, LP, FFP and packed red blood cells (PRBCs) in a ratio of 1:1, or 1:1 LP and PRBCs. MAIN OUTCOME MEASURES: Residual clotting activity of LP after reconstitution, swine mortality, hemodynamic measures, total blood loss, coagulation profiles, and inflammatory measures. RESULTS: Lyophilization decreased clotting factor activity by an average of 14%. Survival and heart rate were similar between all groups. Swine resuscitated with LP had equivalent or higher mean arterial pressures. Swine treated with LP had similar coagulation profiles, plasma lactate levels, and postinjury blood loss compared with those treated with FFP. Swine treated with 1:1 FFP-PRBCs were similar to those treated with 1:1 LP-PRBCs. Resuscitation with LP resulted in a reduction in postresuscitation interleukin 6 expression compared with resuscitation with FFP. CONCLUSIONS:
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Authors | Nicholas Spoerke, Karen Zink, S David Cho, Jerome Differding, Patrick Muller, Ayhan Karahan, Jill Sondeen, John B Holcomb, Martin Schreiber |
Journal | Archives of surgery (Chicago, Ill. : 1960)
(Arch Surg)
Vol. 144
Issue 9
Pg. 829-34
(Sep 2009)
ISSN: 1538-3644 [Electronic] United States |
PMID | 19797107
(Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)
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Chemical References |
- Antioxidants
- Plasma Substitutes
- Ascorbic Acid
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Topics |
- Animals
- Antioxidants
(administration & dosage)
- Ascorbic Acid
(administration & dosage)
- Disease Models, Animal
- Disseminated Intravascular Coagulation
(therapy)
- Fluid Therapy
- Freeze Drying
- Plasma
- Plasma Substitutes
(administration & dosage)
- Resuscitation
(methods)
- Shock, Hemorrhagic
(physiopathology, therapy)
- Swine
- Wounds and Injuries
(physiopathology, therapy)
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