Harnessing the physiology of lymphopenia to support adoptive immunotherapy in lymphoreplete hosts.

Abstract

Lymphopenia enhances the effectiveness of adoptive immunotherapy by facilitating expansion of transferred T cells but also limits the T-cell repertoire available to mediate immune responses and, in humans, is associated with chronic immune dysfunction. Previous studies concluded that lymphopenia augments adoptive immunotherapy by diminishing Tregs and increasing homeostatic cytokines. We sought to determine whether targeted therapies that replicate the physiology of lymphopenia in lymphoreplete hosts could provide a similarly supportive milieu. Pmel-1 T cells were transferred to B16-bearing lymphopenic versus lymphoreplete mice receiving alphaCD25 and/or recombinant human interleukin-7. Although CD25-based Treg depletion was inefficient because of peripheral expansion of CD4+CD25-FOXP3+ cells, outcomes were better in alphaCD25-treated lymphoreplete hosts than in lymphopenic hosts, and adoptive immunotherapy was most effective in lymphoreplete hosts receiving alphaCD25 plus recombinant human interleukin-7. Lymphopenic hosts supported increased proliferation of adoptively transferred antigen-specific T cells, but cells transferred to lymphoreplete recipients receiving targeted therapies showed superior function. Further, determinant spreading was substantial in lymphoreplete hosts but absent in lymphopenic hosts. These results demonstrate that targeted therapies delivered to mimic the "physiology of lymphopenia" enhance the efficacy of adoptive immunotherapy in lymphoreplete hosts and provide a potentially superior alternative to the induction of lymphopenia.

• Authors: Yongzhi Cui, Hua Zhang, Joanna Meadors, Rita Poon, Martin Guimond, Crystal L Mackall
• Journal: Blood (Blood) Vol. 114 Issue 18 Pg. 3831-40 (Oct 29 2009) ISSN: 1528-0020 [Electronic] United States
• PMID: 19704119 (Publication Type: Journal Article, Research Support, N.I.H., Intramural)

Chemical References

• IL7 protein, human
• Il2ra protein, mouse
• Interleukin-2 Receptor alpha Subunit
• Interleukin-7

Topics

• Adoptive Transfer
• Animals
• Cell Proliferation (drug effects)
• Humans
• Interleukin-2 Receptor alpha Subunit (immunology)
• Interleukin-7 (immunology, pharmacology)
• Lymphopenia (immunology, physiopathology, therapy)
• Mice
• Mice, Knockout
• T-Lymphocytes, Regulatory (immunology, transplantation)