Expression of periostin in the serum of NSCLC and its function on proliferation and migration of human lung adenocarcinoma cell line (A549) in vitro.

Abstract

Periostin is over expressed in many epithelial malignant cancers, including lung cancer, breast cancer, ovarian cancer and colon cancer. It is related with the progression and migration of breast and ovarian cancer cells in vitro. The aim of this study was to investigate the serum level of periostin in non-small cell lung cancer (NSCLC) and its relationship with established biological and prognostic factors by enzyme-linked-immunosorbent serologic assay. We also observe the function of periostin on the proliferation and migration of human lung adenocarcinoma cell line (A549) and discuss the mechanism. The mean value for serum periostin (POSTN) was elevated in NSCLC patients (242.84 + or - 5.33 pg/ml) compared to the normal healthy volunteers (215.66 + or - 11.67 pg/ml) (p = 0.030). The serum level of periostin of NSCLC patients had no connection with gender, age, pathological type, TNM stage, lymph node status, tumor size and invasiveness. We constructed a plasmid named pEGFP-N1/POSTN expressing full-length human periostin. Transfecting the plasmid to A549 cells and periostin was efficiently expressed in transfected A549 cells. Our data showed that periostin could promote the proliferation and migration of A549 cells by inducing vimentin and N-cadherin expression and downregulating E-cadherin expression. These results strongly suggest that periostin is a novel molecular which play an important role during the progression and development of NSCLC.

• Authors: Lingzhi Hong, Huiming Sun, Xiaojing Lv, Di Yang, Jinan Zhang, Yi Shi
• Journal: Molecular biology reports (Mol Biol Rep) Vol. 37 Issue 5 Pg. 2285-93 (Jun 2010) ISSN: 1573-4978 [Electronic] Netherlands
• PMID: 19688273 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Cell Adhesion Molecules
• DNA, Recombinant
• POSTN protein, human
• RNA, Messenger

Topics

• Aged
• Biomarkers, Tumor
• Carcinoma, Non-Small-Cell Lung (blood, genetics, pathology)
• Case-Control Studies
• Cell Adhesion Molecules (blood)
• Cell Line, Tumor
• Cell Movement
• Cell Proliferation
• DNA, Recombinant (genetics)
• Enzyme-Linked Immunosorbent Assay
• Epithelium (metabolism, pathology)
• Female
• Gene Expression Regulation, Neoplastic
• Humans
• Lung Neoplasms (blood, genetics, pathology)
• Male
• Mesoderm (metabolism, pathology)
• Microscopy, Fluorescence
• Middle Aged
• Plasmids (genetics)
• RNA, Messenger (genetics, metabolism)
• Transfection
• Wound Healing