Abstract | PURPOSE: METHODS: A total of 29 CC, 19 adjacent non- tumor-containing tissue and 15 normal liver specimens were used for identification of gene methylation status by methylation-specific polymerase chain reaction. RESULTS: CONCLUSIONS: Taken together, these results suggest that loss of the integrin alpha4 gene is caused by aberrant DNA methylation of the 5'-CpG island site of the gene, and methylation of the integrin alpha4 gene can be a useful marker of metastasis of CC.
|
Authors | Kyung-Ok Uhm, Jung Ok Lee, Yun Mi Lee, Eun Soo Lee, Hyeon Soo Kim, Sun-Hwa Park |
Journal | Journal of cancer research and clinical oncology
(J Cancer Res Clin Oncol)
Vol. 136
Issue 2
Pg. 187-94
(Feb 2010)
ISSN: 1432-1335 [Electronic] Germany |
PMID | 19655168
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Antimetabolites, Antineoplastic
- Biomarkers, Tumor
- Enzyme Inhibitors
- Integrin beta1
- Paxillin
- RNA, Small Interfering
- Integrin alpha4
- Methyltransferases
- Azacitidine
|
Topics |
- Adult
- Aged
- Antimetabolites, Antineoplastic
(pharmacology)
- Azacitidine
(pharmacology)
- Base Sequence
- Bile Duct Neoplasms
(genetics, pathology)
- Bile Ducts, Intrahepatic
- Biomarkers, Tumor
(genetics)
- Cell Line, Tumor
- Cholangiocarcinoma
(genetics, secondary)
- CpG Islands
(genetics)
- DNA Methylation
(drug effects)
- Enzyme Inhibitors
(pharmacology)
- Female
- Gene Expression Regulation, Neoplastic
(drug effects)
- Gene Silencing
- Humans
- Integrin alpha4
(drug effects, genetics)
- Integrin beta1
(genetics)
- Lymphatic Metastasis
- Male
- Methyltransferases
(antagonists & inhibitors)
- Middle Aged
- Molecular Sequence Data
- Neoplasm Invasiveness
- Paxillin
(metabolism)
- Phosphorylation
- RNA, Small Interfering
- Reverse Transcriptase Polymerase Chain Reaction
- Sequence Analysis, DNA
- Transfection
|