Expression of DBC1 and SIRT1 is associated with poor prognosis of gastric carcinoma.

Abstract

PURPOSE:

SIRT1 (silent mating-type information regulation 2 homologue 1) expression has been reported to predict poor survival in some cancers. We therefore investigated the expression levels of SIRT1 and its negative regulator, DBC1 (deleted in breast cancer 1), in gastric cancer patients.

EXPERIMENTAL DESIGN:

We evaluated immunohistochemical expression of DBC1, SIRT1, and p53 using 3-mm tumor cores from 177 gastric cancer patients for tissue microarray.

RESULTS:

Positive expressions of DBC1 and SIRT1 were seen in 62% (109 of 177) and in 73% (130 of 177) of patients, respectively. Expression of DBC1 was significantly correlated with tumor stage (P = 0.007), lymph node metastasis (P < 0.001), tumor invasion (P = 0.001), venous invasion (P = 0.001), histologic types (P < 0.001), p53 expression (P < 0.001), and SIRT1 expression (P < 0.001). SIRT1 expression was also significantly correlated with tumor stage (P < 0.001), lymph node metastasis (P < 0.001), tumor invasion (P < 0.001), histologic types (P < 0.001), and p53 expression (P = 0.001). In addition, expression of DBC1 was significantly associated with shorter overall survival and relapse-free survival by univariate analysis (P < 0.001 and P < 0.001, respectively). SIRT1 expression was also significantly associated with shorter overall survival and relapse-free survival by univariate analysis (P = 0.001 and P = 0.001, respectively). Multivariate analysis showed that tumor stage and expression of DBC1 were independent prognostic factors significantly associated with overall survival and relapse-free survival.

CONCLUSION:

This study shows that expression of DBC1 and SIRT1 is a significant prognostic indicator for gastric carcinoma patients.

• Authors: Eun Jung Cha, Sang Jae Noh, Keun Sang Kwon, Chan Young Kim, Byung-Hyun Park, Ho Sung Park, Ho Lee, Myoung Ja Chung, Myoung Jae Kang, Dong Geun Lee, Woo Sung Moon, Kyu Yun Jang
• Journal: Clinical cancer research : an official journal of the American Association for Cancer Research (Clin Cancer Res) Vol. 15 Issue 13 Pg. 4453-9 (Jul 01 2009) ISSN: 1557-3265 [Electronic] United States
• PMID: 19509139 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adaptor Proteins, Signal Transducing
• CCAR2 protein, human
• Tumor Suppressor Proteins
• SIRT1 protein, human
• Sirtuin 1
• Sirtuins

Topics

• Adaptor Proteins, Signal Transducing (genetics)
• Adult
• Aged
• Aged, 80 and over
• Carcinoma (diagnosis, genetics, mortality, pathology)
• Case-Control Studies
• Female
• Follow-Up Studies
• Gene Expression Regulation, Neoplastic
• Humans
• Male
• Middle Aged
• Prognosis
• Recurrence
• Sirtuin 1
• Sirtuins (genetics)
• Stomach Neoplasms (diagnosis, genetics, mortality, pathology)
• Survival Analysis
• Tumor Suppressor Proteins (genetics)