Microinjection analysis of envelope-glycoprotein messenger activities of avian leukosis viral RNAs.

Abstract

Virion RNA from the avian leukosis virus Rous-associated virus 2 (RAV-2) and poly(A)-containing RNAs from RAV-2-infected chick embryo fibroblasts were microinjected into fibroblasts transformed by the Bryan high-titer strain of Rous sarcoma virus (RSV), which is deficient in viral envelope glycoprotein. Production of infectious RSV following these injections depended upon the viral envelope-messenger activity of the injected RNA. This system constituted a sensitive and rigorous assay system for viral envelope-messenger RNA. It was found that 21S mRNA from RAV-2-infected cells expressed the highest activity, while 35S mRNA expressed comparatively little. In addition, RAV-2-virion RNA expressed little messenger activity. The rate of formation of infectious RSV following 21S mRNA injections reached a peak near 9 hr, which was followed by a rapid decline. Evidence has been obtained that a small fraction of both 35S virion RNA and 35S mRNA from virus-infected cells was encapsulated into virus particles following their injection into virus-producing cells.

• Authors: D W Stacey, V G Allfrey, H Hanafusa
• Journal: Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 74 Issue 4 Pg. 1614-8 (Apr 1977) ISSN: 0027-8424 [Print] United States
• PMID: 193109 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Glycoproteins
• Membrane Proteins
• RNA, Messenger
• RNA, Viral
• Poly A

Topics

• Animals
• Avian Leukosis Virus
• Chick Embryo
• Fibroblasts (metabolism)
• Glycoproteins (biosynthesis)
• Kinetics
• Membrane Proteins (biosynthesis)
• Microinjections
• Poly A
• Protein Biosynthesis
• RNA, Messenger (metabolism)
• RNA, Viral (metabolism)
• Virus Replication