Inhibitory effect of recombinant IL-25 on the development of dextran sulfate sodium-induced experimental colitis in mice.

Abstract

The role of interleukin 25 (IL-25) in a number of human diseases still has not been extensively studied, here we attempt to evaluate the role of recombinant IL-25 (rIL-25) in the development of dextran sulfate sodium (DSS)-induced experimental colitis. Acute colitis was induced in female C57BL/6 mice by oral administration of 2.5% DSS in drinking water ad libitum. At the same time as the start of DSS exposure, mice were injected intraperitoneally with 0.4 microg of rIL-25 or PBS. Then disease activity index (DAI), histological changes and survival rate were observed. The levels of IL-17, IL-23, and TGF-beta1 in colon tissues were determined by ELISA, and the production of IL-17 by CD4(+)/CD8(+) T cells was detected by intracellular flow cytometry. In contrast to the DSS treated mice, DSS + rIL-25 treated mice displayed a lower DAI, limited histological changes and prolonged survival. The levels of IL-23 and TGF-beta1 were significantly elevated in the DSS + rIL-25 treated mice compared to the DSS treated mice. There was no significant difference in the production of IL-17 in colon tissues and CD4(+)/CD8(+) T cells between the DSS + rIL-25 treated mice and DSS treated mice. Our findings suggest the role of IL-25 in inhibiting development and progression of acute colitis in DSS-induced mouse colitis model.

• Authors: S S Salum Mchenga, Danan Wang, Cheng Li, Fengping Shan, Changlong Lu
• Journal: Cellular & molecular immunology (Cell Mol Immunol) Vol. 5 Issue 6 Pg. 425-31 (Dec 2008) ISSN: 2042-0226 [Electronic] China
• PMID: 19118508 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Interleukin-17
• Interleukin-23
• Recombinant Proteins
• Transforming Growth Factor beta1
• Dextran Sulfate

Topics

• Animals
• CD4-Positive T-Lymphocytes (drug effects, immunology)
• CD8-Positive T-Lymphocytes (drug effects, immunology)
• Colitis (drug therapy, pathology)
• Dextran Sulfate
• Disease Progression
• Female
• Flow Cytometry
• Interleukin-17 (metabolism, pharmacology, therapeutic use)
• Interleukin-23 (metabolism)
• Kaplan-Meier Estimate
• Mice
• Mice, Inbred C57BL
• Recombinant Proteins (pharmacology, therapeutic use)
• Transforming Growth Factor beta1 (metabolism)