Comparison of SYBR Green I and TaqMan real-time PCR formats for the analysis of her2 gene dose in human breast tumors.

Abstract

We compared two technologies of real-time PCR (with the use of fluorescent SYBR Green I dye and specific TaqMan probe) for quantification of the dose of her2 gene in breast tumors. The maximum increase in the gene dose in TaqMan and SYBR Green I analyses was 10- and 5-fold, respectively. In was found that TaqMan and SYBR Green I technologies allow detection of the matrix in amounts corresponding to 1-100 and 2.5-40.0 ng genomic DNA, respectively. Tenfold increase in the gene dose leads to incorrect evaluation of multiplication ratio in the SYBR Green I analysis. These results suggest that TaqMan technology is more preferable for correct evaluation of her2 gene dose.

• Authors: N U Matsenko, V S Rijikova, S P Kovalenko
• Journal: Bulletin of experimental biology and medicine (Bull Exp Biol Med) Vol. 145 Issue 2 Pg. 240-4 (Feb 2008) ISSN: 0007-4888 [Print] United States
• PMID: 19023979 (Publication Type: Evaluation Study, Journal Article)

Chemical References

• Benzothiazoles
• Diamines
• Fluorescent Dyes
• Organic Chemicals
• Quinolines
• SYBR Green I
• ERBB2 protein, human
• Receptor, ErbB-2

Topics

• Benzothiazoles
• Breast Neoplasms (genetics)
• Diamines
• Female
• Fluorescent Dyes (metabolism)
• Gene Dosage
• Humans
• Organic Chemicals (metabolism)
• Polymerase Chain Reaction (instrumentation, methods)
• Quinolines
• Receptor, ErbB-2 (genetics)