Apoptotic effect of CKD-602 (Camtobell) on oral squamous cell carcinoma cell lines.

Abstract

The purposes of this study were to measure the cytotoxic effect of CKD-602 on oral squamous cell carcinoma (OSCC) cell lines, to evaluate the apoptotic aspect of dead cells, and to identify the signaling molecules involved in apoptosis. The human OSCC cell lines A253, HSC-3 and KB were treated with CKD-602. The apoptotic proportion of the cells was analyzed using flow cytometry. The expression of Bax, Bcl-2, and p53 were detected by western blotting analysis. CKD-602 showed excellent cytotoxicity to the OSCC cell lines. Most cell death was attributed to apoptosis rather than necrosis. CKD-602 induced the down-regulation of Bcl-2 in A253 and HSC-3 cells, and p53 was expressed in the KB cell line after treatment with CKD-602.

• Authors: Yong-Ju Ok, Hoon Myoung, Young-Kyun Kim, Jong-Ho Lee, Myung-Jin Kim, Pil-Young Yun
• Journal: Oral oncology (Oral Oncol) Vol. 45 Issue 3 Pg. 266-72 (Mar 2009) ISSN: 1879-0593 [Electronic] England
• PMID: 18715816 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents, Phytogenic
• Peptide Fragments
• Proto-Oncogene Proteins c-bcl-2
• Topoisomerase I Inhibitors
• Tumor Suppressor Protein p53
• bcl-2-Associated X Protein
• p53 protein (325-355), human
• belotecan
• Camptothecin

Topics

• Antineoplastic Agents, Phytogenic (pharmacology)
• Apoptosis (drug effects)
• Camptothecin (analogs & derivatives, pharmacology)
• Carcinoma, Squamous Cell (drug therapy, genetics, metabolism)
• Cell Line, Tumor
• Humans
• Mouth Mucosa
• Mouth Neoplasms (drug therapy, genetics, metabolism)
• Peptide Fragments
• Proto-Oncogene Proteins c-bcl-2 (metabolism)
• Submandibular Gland Neoplasms (drug therapy, genetics, metabolism)
• Tongue Neoplasms (drug therapy, genetics, metabolism)
• Topoisomerase I Inhibitors
• Tumor Suppressor Protein p53 (biosynthesis)
• bcl-2-Associated X Protein (metabolism)