Transcriptional analysis of estrogen receptor alpha variant mRNAs in colorectal cancers and their matched normal colorectal tissues.

Abstract

Estrogen is involved in suppression of colorectal cancer development and exerts its function via estrogen receptors alpha, beta and their splicing variants. Whether the recently indentified ER-alpha splicing variants, ER-alpha36 and ER-alpha46, play a role in colorectal cancer development is unknown. In this study, we quantified the mRNA copy numbers of wild type ER-alpha (ER-alpha66), ER-alpha46 and ER-alpha36 in 35 colorectal cancers and their matched normal colorectal tissues by quantitative real-time PCR assay, and correlated their mRNA levels with the clinicopathological properties of the tumors. We found that ER-alpha66, ER-alpha46 and ER-alpha36 mRNAs were coexpressed in all colorectal cancers and their matched normal tissues. The decreased mRNA levels of ER-alpha36 and ER-alpha46 whereas no difference of ER-alpha66 mRNA was observed in colorectal cancers compared to their matched normal tissues. Moreover, change in the expression of ER-alpha36 mRNA level was correlated with Dukes' stage of the tumor and the lymph node metastasis. ER-alpha36 mRNA was decreased significantly in Dukes' C+D compared to Dukes' A+B stage tumors (P=0.017), and the expression of ER-alpha36 mRNA in N(1)/N(2) was lower than that in N(0) lymph node metastasis (P=0.049). So ER-alpha36 and ER-alpha46 might be implicated in the development and progression of colorectal cancers.

• Authors: Haiping Jiang, Rongyue Teng, Qi Wang, Xing Zhang, Haohao Wang, Zhaoyi Wang, Jiang Cao, Lisong Teng
• Journal: The Journal of steroid biochemistry and molecular biology (J Steroid Biochem Mol Biol) Vol. 112 Issue 1-3 Pg. 20-4 (Nov 2008) ISSN: 0960-0760 [Print] England
• PMID: 18703141 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Estrogen Receptor alpha
• RNA, Messenger
• RNA, Neoplasm

Topics

• Adult
• Alternative Splicing
• Colon (metabolism)
• Colorectal Neoplasms (metabolism)
• Estrogen Receptor alpha (metabolism)
• Female
• Humans
• Lymph Nodes (metabolism)
• Male
• Middle Aged
• RNA, Messenger (metabolism)
• RNA, Neoplasm (metabolism)
• Rectum (metabolism)