Microcarrier culture of COS-1 cells producing Japanese encephalitis and dengue virus serotype 4 recombinant virus-like particles.

Abstract

Two stably transfected COS-1 cell lines that secrete recombinant Japanese encephalitis and dengue virus serotype 4 virus-like particles (VLPs) have been adapted to grow on Cytodex 3 microcarriers in an orbital shaker flask platform. The VLPs are used as antigens in diagnostic enzyme-linked immunosorbent assays to detect anti-arboviral IgM and IgG antibodies in human serum samples. Converting from a stationary flask batch system to a microcarrier fed-batch system has led to increases in antigen concentration while decreasing costs by reducing the amount of cell culture medium, disposables, and labor. The cell culture longevity was increased by 48 days in this optimized system, which may be due to a continual supply of nutrients resulting in prolonged survival of the cells on the microcarrier surface. An initial trial using a serum-free medium with this cell line was promising and may lead to reductions in cost, while reducing the variability between batches introduced by fetal bovine serum.

• Authors: Jason O Velez, Brandy J Russell, Holly R Hughes, Gwong-Jen J Chang, Barbara W Johnson
• Journal: Journal of virological methods (J Virol Methods) Vol. 151 Issue 2 Pg. 230-236 (Aug 2008) ISSN: 0166-0934 [Print] Netherlands
• PMID: 18586334 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antigens, Viral

Topics

• Animals
• Antigens, Viral (analysis)
• COS Cells
• Chlorocebus aethiops
• Dengue Virus (classification, isolation & purification, physiology)
• Encephalitis Virus, Japanese (isolation & purification, physiology)
• Serotyping
• Virus Replication