A bicistronic baculovirus vector for transient and stable protein expression in mammalian cells.

Abstract

Baculoviruses are widely used for protein production in insect cells, and their potential for gene transfer to mammalian cells is increasingly being recognized. Here we describe a baculovirus vector with a bicistronic mammalian expression cassette and demonstrate its suitability for efficient transient and stable protein expression in human glioblastoma cells. Bicistronic baculovirus vectors are safe, cost efficient, and easy to produce; thus, they represent an excellent gene transfer system for mammalian cells.

• Authors: Andreas Lackner, Kathrin Genta, Herwig Koppensteiner, Irene Herbacek, Klaus Holzmann, Sabine Spiegl-Kreinecker, Walter Berger, Michael Grusch
• Journal: Analytical biochemistry (Anal Biochem) Vol. 380 Issue 1 Pg. 146-8 (Sep 01 2008) ISSN: 1096-0309 [Electronic] United States
• PMID: 18541133 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Proteins

Topics

• Animals
• Baculoviridae (genetics)
• Cell Line, Tumor
• Gene Expression Regulation
• Gene Transfer Techniques
• Genetic Vectors (genetics)
• Glioblastoma (genetics, metabolism, pathology)
• Humans
• Proteins (genetics)
• Time Factors
• Transduction, Genetic