Abstract |
Shortage of oxygen is one of the prime stress conditions in tissues. In this study, we looked for microRNAs expressed during hypoxia and showed that miR-210 expression was upregulated in response to hypoxia in vitro and in vivo. An active form of the HIF-1alpha induced the expression of miR-210, showing the involvement of the HIF-1 signaling pathway in miR-210 gene transcription. Furthermore, miR-210 was shown to bind to the predicted target sites of ephrin-A3 or neuronal pentraxin 1, causing repression in luciferase reporter activity. Contrary to the microRNA-mediated repression hypothesis, ephrin-A3 was expressed at very high levels in post-ischemic mouse hippocampus in vivo. Thus, the regulatory effects of miR-210 on its targets in vivo need to be further characterized.
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Authors | Kati Pulkkinen, Tarja Malm, Mikko Turunen, Jari Koistinaho, Seppo Ylä-Herttuala |
Journal | FEBS letters
(FEBS Lett)
Vol. 582
Issue 16
Pg. 2397-401
(Jul 09 2008)
ISSN: 0014-5793 [Print] England |
PMID | 18539147
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- 3' Untranslated Regions
- Ephrin-A3
- MicroRNAs
- Nerve Tissue Proteins
- RNA, Messenger
- neuronal pentraxin
- C-Reactive Protein
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Topics |
- 3' Untranslated Regions
(chemistry)
- Animals
- Brain Ischemia
(metabolism)
- C-Reactive Protein
(genetics, metabolism)
- Cell Hypoxia
- Cell Line, Tumor
- Cells, Cultured
- Ephrin-A3
(genetics, metabolism)
- Gene Expression Regulation
- Humans
- Mice
- MicroRNAs
(biosynthesis, chemistry, metabolism)
- Nerve Tissue Proteins
(genetics, metabolism)
- RNA, Messenger
(metabolism)
- Rabbits
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