Abstract | OBJECTIVE: To investigate the protective effect of L-carnitine (LC) against deleterious substances present in the peritoneal fluid (PF) of patients with endometriosis, which may affect the oocyte cytoskeleton and embryogenesis. DESIGN: Experimental study. SETTING: Research embryology laboratory at an academic hospital. PATIENT(S): Frozen metaphase II mouse oocytes and embryos. INTERVENTION(S): MAIN OUTCOME MEASURE(S): Protective effect of LC on oocytes and embryos. RESULT(S): Incubation of the oocytes and the embryos with PF from patients with endometriosis statistically significantly damaged the oocyte microtubules and chromosomes and increased embryo apoptosis compared with controls. Incubation with LC (0.6 mg/mL) statistically significantly improved microtubule and chromosome structure and decreased the level of embryo apoptosis. CONCLUSION(S): We propose the use of LC as a supplement in patients with endometriosis, a novel approach that may help improve in vitro fertilization outcome in these patients.
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Authors | Gihan Mansour, Hussein Abdelrazik, Rakesh K Sharma, Emad Radwan, Tommaso Falcone, Ashok Agarwal |
Journal | Fertility and sterility
(Fertil Steril)
Vol. 91
Issue 5 Suppl
Pg. 2079-86
(May 2009)
ISSN: 1556-5653 [Electronic] United States |
PMID | 18394615
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
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Topics |
- Animals
- Apoptosis
(drug effects)
- Ascitic Fluid
(pathology)
- Carnitine
(therapeutic use)
- Embryo, Mammalian
(drug effects, pathology, physiology)
- Endometriosis
(pathology)
- Female
- Humans
- Laparoscopy
- Mice
- Microscopy, Confocal
- Microtubules
(drug effects, physiology)
- Oocytes
(drug effects, pathology)
- Pregnancy
- Sterilization Reversal
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