Continuous subcutaneous
insulin infusion (CSII) is a commonly used, safe intensive
insulin therapy method effective in maintaining normoglycaemia. The disadvantage of CSII are skin
infections of the
catheter injection site. The aim of the study was to gain insight on the colonization of subcutaneous
insulin pump
catheters by skin flora and to investigate the correlation between Staphylococcus aureus carrier state (presence in the nose), its presence on the skin and
catheter. 141
catheters obtained from 94 children with T1DM and CSII were examined using the semi quantitative culture technique of Maki. The result was positive in 34 examinations (24.1%) in 30 children (31.9%). Most often
coagulase negative staphylococci were isolated (30), mainly Staphylococcus epidermidis, 1/3 of the staphylococci were methicillin resistant. S. aureus was detected in 7 examinations in 6 children. S. aureus carrier state was proved in 31.9% of all examined patients, more often in children with a positive
catheter culture (41.4%), there were no MRSA. No correlation between S. aureus carrier state and
catheter colonization was shown. Statistically significant correlations between:
coagulase negative staphylococci presence, including the methicillin resistant strains, on the skin and on the
catheter surface (p< 0.0001);
glycosylated hemoglobin (HbA1c) and bacteria
catheter colonization (p = 0.0335) were observed. Subcutaneous
catheter colonization by microorganisms often occurs in CSII. Microorganisms found on the skin are the most frequent cause of the subcutaneous
catheter infection.