Determination of terephthalic acid isopropylamide in urine with a liquid chromatography/mass spectrometry (LC/MS) method.

Abstract

A sensitive and simple liquid chromatography/mass spectrometry (LC/MS) method was developed for the determination of terephthalic acid isopropylamide, the final metabolite of procarbazine in human urine. A solid-phase extraction with C(18) cartridges was used followed by LC/MS with a single mass spectrometer (SSQ 7000 from Finnigan). Terephthalic acid isobutylamide was the internal standard. The quantification limit was 30 ng/mL in urine (6 x noise). This assay was applied for drug monitoring of terephthalic acid isopropylamide in urine after oral administration of procarbazine in children and adolescents with Hodgkin lymphomas.

• Authors: F Baumann, C Mauz-Körholz, D Clauss, S Borrmann, A Giannis, N Merkel, D Körholz, R Preiss
• Journal: Journal of clinical laboratory analysis (J Clin Lab Anal) Vol. 22 Issue 1 Pg. 21-8 ( 2008) ISSN: 0887-8013 [Print] United States
• PMID: 18200575 (Publication Type: Journal Article)
• Copyright: (c) 2008 Wiley-Liss, Inc.

Chemical References

• Phthalic Acids
• Procarbazine
• terephthalic acid
• terephthalic acid isopropylamide

Topics

• Adolescent
• Chromatography, Liquid (methods)
• Drug Monitoring
• Drug Stability
• Female
• Humans
• Kinetics
• Male
• Mass Spectrometry (methods)
• Phthalic Acids (chemistry, urine)
• Procarbazine (administration & dosage, chemistry, metabolism, pharmacology)