Induction of cell death by combination treatment with cisplatin and 5-fluorouracil in a human oral squamous cell carcinoma cell line.

Abstract

The possible apoptosis-inducing activity of several sequential treatments of cisplatin (CDDP) and 5-fluorouracil (5-FU) against the human oral squamous cell carcinoma HSC-2 cell line was investigated. The following three combination treatments (CT) were used: simultaneous treatment with CDDP and 5-FU (for 72 hours) (CT-1), CDDP treatment (24 hours) followed by 5-FU treatment (48 hours) (CT-2) and 5-FU treatment (24 hours) followed by CDDP treatment (48 hours) (CT-3). CT-1 produced the highest cytotoxicity, followed by CT-3 and CT-2. No treatment induced any detectable internucleosomal DNA fragmentation, and caspase-3,-8 and -9 were activated to a much lesser extent than that attained using actinomycin D. High-performance liquid chromatography analysis demonstrated that 5-FU, as well as CT-1 and CT-2, preferentially reduced the intracellular concentration of putrescine. These results suggest that simultaneous treatment with CDDP and 5-FU induces lower level of apoptotic cell death in HSC-2 cells.

• Authors: Masahiko Okamura, Masaki Kobayashi, Fumika Suzuki, Jun Shimada, Hiroshi Sakagami
• Journal: Anticancer research (Anticancer Res) 2007 Sep-Oct Vol. 27 Issue 5A Pg. 3331-7 ISSN: 0250-7005 [Print] Greece
• PMID: 17970078 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biogenic Polyamines
• Isoenzymes
• Caspases
• Cisplatin
• Fluorouracil

Topics

• Antineoplastic Combined Chemotherapy Protocols (administration & dosage)
• Apoptosis (drug effects)
• Biogenic Polyamines (metabolism)
• Carcinoma, Squamous Cell (drug therapy, metabolism, pathology)
• Caspases (metabolism)
• Cell Line, Tumor
• Cisplatin (administration & dosage)
• DNA Fragmentation
• Drug Administration Schedule
• Enzyme Activation
• Fluorouracil (administration & dosage)
• HL-60 Cells
• Humans
• Isoenzymes (metabolism)
• Mouth Neoplasms (drug therapy, metabolism, pathology)