Effectiveness of narrow-band ultraviolet-B phototherapy for prevention of intimal hyperplasia in a rat carotid balloon injury model.

Abstract

BACKGROUND AND OBJECTIVE:

Narrow-band ultraviolet-B light (NBUVB) (313 nm) is known to have anti-proliferative effects, implying a potential treatment for intimal hyperplasia, but it remains to be ascertained. We assessed the effects of NBUVB irradiation for prevention of intimal hyperplasia.

STUDY DESIGN/MATERIALS AND METHODS:

The rat carotid arteries were irradiated with NBUVB after balloon injury (BI), and the degree of intimal hyperplasia was histopathologically assessed. The anti-proliferative effects using cultured human smooth muscle cells were evaluated by flow cytometry and immunoblot analysis.

RESULTS:

NBUVB (0.3-4.5 J/cm(2)) irradiation immediately after BI reduced the degree of intimal hyperplasia at 14 and 28 days after BI (P<0.001) without any obvious complications. Neither an increase in the number of medial cells nor upregulation of proliferating cell nuclear antigen was observed in the irradiated arteries. NBUVB irradiation at 2 or 14 days after BI significantly suppressed further intimal hyperplasia (P<0.01). NBUVB-irradiated cultured cells showed inhibited proliferation involved with G(1) and G(2)/M arrests. Increased expression of p53 and inhibition of retinoblastoma protein (pRB) phosphorylation were also seen in the NBUVB-irradiated cells.

CONCLUSIONS:

These data suggest that NBUVB irradiation is an effective method for preventing intimal hyperplasia. The anti-proliferative effect is partly due to the cell cycle arrest caused by p53 expression and inhibited pRB phosphorylation.

• Authors: Shinya Kohyama, Yuji Morimoto, Kanji Nakai, Tatsumi Kaji, Aya Tokumaru, Hiroshi Nawashiro, Katsuji Shima, Yasushi Satoh, Kunio Takishima, Makoto Kikuchi, Hirotaka Matsuo
• Journal: Lasers in surgery and medicine (Lasers Surg Med) Vol. 39 Issue 8 Pg. 659-66 (Sep 2007) ISSN: 0196-8092 [Print] United States
• PMID: 17886279 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: 2007 Wiley-Liss, Inc

Topics

• Animals
• Blotting, Western
• Carotid Artery Injuries (pathology, prevention & control)
• Carotid Artery, Common (pathology)
• Catheterization
• Cell Proliferation
• Disease Models, Animal
• Flow Cytometry
• Hyperplasia
• Male
• Rats
• Rats, Sprague-Dawley
• Tunica Intima (injuries, pathology)
• Ultraviolet Therapy