Abstract |
We have studied the effects of cyclosporine A (CsA) on basal and bovine parathyroid hormone (1-34) (bPTH)-stimulated bone resorption by osteoclasts in 24-hour cultures of chick long bone cells. At a high concentration (10 micrograms/ml), CsA had a cytotoxic effect on both osteoclasts and mononuclear cells in the culture. At 1 microgram/ml, CsA inhibited basal and bPTH-stimulated bone resorption but was not cytotoxic over 24 hours. We also studied the binding of bPTH to the osteoblastic cell line, Saos-2, and chick long bone cells in suspension culture. CsA inhibited bPTH binding in Saos-2 in a dose-dependent manner; inhibition of binding was also observed in chick bone cells. The effects of CsA on osteoclast viability and resorptive function may be due to a direct effect on the osteoclasts and/or to an interaction with the nonosteoclastic cell population in the culture.
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Authors | M H Chowdhury, V Shen, D W Dempster |
Journal | Calcified tissue international
(Calcif Tissue Int)
Vol. 49
Issue 4
Pg. 275-9
(Oct 1991)
ISSN: 0171-967X [Print] United States |
PMID | 1760772
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Parathyroid Hormone
- Peptide Fragments
- parathyroid hormone (1-34), bovine
- Cyclosporine
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Topics |
- Analysis of Variance
- Animals
- Bone Resorption
- Cell Count
- Cell Line
- Cell Survival
(drug effects)
- Cells, Cultured
- Chickens
- Cyclosporine
(pharmacology)
- Osteoclasts
(drug effects, metabolism, physiology)
- Parathyroid Hormone
(metabolism, pharmacology)
- Peptide Fragments
(metabolism, pharmacology)
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