Use of amaranthus leucocarpus lectin to differentiate cervical dysplasia (CIN).

Abstract

Alterations in O-glycosylation of proteins in cell surfaces can originate disorder in cellular function, as well as in cell transformation and tumoral differentiation. In this work, we investigate changes in O-glycosylation in cervical intraepithelial dysplasia (CIN) at different stages of differentiation (CIN I, CIN II, and CIN III) using lectins specific for O-glycosidically linked glycans. Twenty cases with CIN I, CIN II, and CIN III dysplasias each, and 20 normal cases were studied by lectin histochemistry and evaluated under optical microscopy. The lectins from Glycine max and Griffonia simplicifolia showed no differences in their recognition pattern among the different CIN stages and normal tissue. Dolichos Biflorus lectin recognized CIN I dysplasia. Lectin from Amaranthus leucocarpus showed increased reactivity in the presence of CIN II dysplasia, compared with CIN I and CIN III. These results suggest that subtle modifications in the O-glycosylation pattern could be considered in diagnosis or prognosis of cervical precancerous stages.

• Authors: Arturo Santaella-Verdejo, Belem Gallegos, Eduardo Pérez-Campos, Pedro Hernández, Edgar Zenteno
• Journal: Preparative biochemistry & biotechnology (Prep Biochem Biotechnol) Vol. 37 Issue 3 Pg. 219-28 ( 2007) ISSN: 1082-6068 [Print] England
• PMID: 17516251 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amaranthus leucocarpus lectin
• Biomarkers, Tumor
• Glycoproteins
• Griffonia simplicifolia lectins
• Plant Lectins
• dolichos biflorus agglutinin

Topics

• Amaranthus (chemistry)
• Biomarkers, Tumor (analysis)
• Biopsy, Needle
• Cell Differentiation
• Dolichos (chemistry)
• Female
• Glycoproteins (analysis)
• Griffonia (chemistry)
• Histocytochemistry
• Humans
• Neoplasm Staging (methods)
• Neoplasms, Squamous Cell (chemistry, pathology)
• Plant Lectins (analysis)
• Protein Binding
• Uterine Cervical Dysplasia (chemistry, diagnosis, pathology)
• Uterine Cervical Neoplasms (chemistry, pathology)