[Effects of phosphatidylcholine therapy after hindlimb ischemia and reperfusion].

Abstract

BACKGROUND:

Microcirculatory dysfunctions and mast cell reactions play important roles in hypoxic tissue injuries. The aims of this study were to characterize the effects of hindlimb ischemia-reperfusion on the periosteal microcirculation and to define the consequences of systemic phosphatidylcholine therapy during this condition.

MATERIALS AND METHODS:

Microcirculatory changes were visualized by means of fluorescence intravital videomicroscopy in anesthetized Wistar rats. 60 min of complete hindlimb ischemia was followed by a 180-min reperfusion in the presence of phosphatidylcholine treatment (50 mg/kg iv; in the second 10 min of reperfusion) or vehicle. Further two groups served as vehicle- or PC-treated sham-operated controls. The proportion of degranulated mast cells and the leukocyte accumulation (myeloperoxidase, MPO assay) were determined in muscle biopsies.

RESULTS:

Ischemia-reperfusion significantly increased the muscle MPO activity (from 14.94 to 63.45 mU/mg) and the proportion of degranulated mast cells (to 82.5%). The periosteal capillary red blood cell velocity (RBCV) and the functional capillary density (FCD) had decreased, while the primary and secondary leukocyte-endothelial cell interactions had increased by the end of reperfusion (rolling from 20.8 to 40.0%, and firm adherence from 254 to 872 mm-2). Phosphatidylcholine treatment decreased the leukocyte rolling and sticking, preserved the FCD and improved the RBCV The mast cell degranulation and MPO activity diminished significantly in the muscle layer.

CONCLUSIONS:

Mast cell degranulation accompanies ischemia-reperfusion-induced periosteal microcirculatory derangement. Systemic phosphatidylcholine treatment affords protection through ameliorating secondary inflammatory reactions.

• Authors: Renáta Varga, László Gera, László Török, József Kaszaki, Andrea Szabó, Katalin Nagy, Mihály Boros
• Journal: Magyar sebeszet (Magy Seb) Vol. 59 Issue 6 Pg. 429-36 (Dec 2006) ISSN: 0025-0295 [Print] Hungary
• Vernacular Title: A foszfatidilkolin kezelés hatása kísérletes vegtag iszkémiát követoen.
• PMID: 17432083 (Publication Type: English Abstract, Journal Article)

Chemical References

• Phosphatidylcholines
• Peroxidase

Topics

• Animals
• Blood Flow Velocity (drug effects)
• Capillaries (drug effects)
• Erythrocytes (drug effects)
• Hindlimb (blood supply)
• Leukocytes (drug effects, enzymology)
• Male
• Mast Cells (drug effects)
• Muscle, Skeletal (drug effects, enzymology, metabolism)
• Peroxidase (drug effects, metabolism)
• Phosphatidylcholines (pharmacology)
• Rats
• Rats, Wistar
• Reperfusion Injury (prevention & control)
• Time Factors
• Video Recording