[Subject diagnostic value of detecting a1pha-fetoprotein variants with a new microspincolumn method in hepatocellular carcinoma].

Abstract

OBJECTIVE:

To evaluate the usefulness of new microspincolumn method for the measurement of a1pha-fetoprotein variant AFP-L3 in differentiation of benign and malignant liver disease and the warming for liver cancer.

METHODS:

AFP-L3 was isolated by using microspincolumn coupled with lens culinaris agglutinin (LCA), AFP and AFP-L3 were determined with chemiluminescent immunoassay, the proportion of AFP-L3 levels AFP-L3(%) were calculated, and the relationship between the elevated AFP-L3(%) levels and benign and malignant liver disease was analyzed.

RESULTS:

The levels of AFP-L3(%) in serum of patients with hepatocellular carcinoma was significantly higher than those in the patients with other liver diseases (P < 0.001). Taking AFP-L3(%) >or= 10% as the diagnostic criteria, the sensitivity for diagnosis of liver cancer was 90.9%.

CONCLUSION:

Detection of AFP-L3 seemed to be of clinical value in diagnosis and differential diagnosis of hepatocellular carcinoma; it may be especially important for identifying patients with hepatocellular carcinoma whose a1pha-fetoprotein level is low.

• Authors: Ai-fang Xu, Miao-chan Wang, Dong-ming Sui, You-hong Yuan, Gang Chen, Guo-qiang Lou
• Journal: Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology (Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi) Vol. 21 Issue 1 Pg. 67-9 (Mar 2007) ISSN: 1003-9279 [Print] China
• PMID: 17429541 (Publication Type: English Abstract, Journal Article)

Chemical References

• alpha-Fetoproteins

Topics

• Adult
• Aged
• Carcinoma, Hepatocellular (blood, diagnosis)
• Diagnosis, Differential
• Female
• Hepatitis, Chronic (blood, diagnosis)
• Humans
• Immunoassay (methods)
• Liver Cirrhosis (blood, diagnosis)
• Liver Neoplasms (blood, diagnosis)
• Luminescent Measurements (methods)
• Male
• Middle Aged
• Sensitivity and Specificity
• Young Adult
• alpha-Fetoproteins (analysis)