Acute perfusion of BMAA in the rat's striatum by in vivo microdialysis.

Abstract

The present study is concerned with the hypothetical toxicity of beta-N-methylamino-L-alanine (BMAA), a compound that has been hypothesized to produce amyotrophic lateral sclerosis/Parkinson-dementia complex. We have used the microdialysis technique to perfused different concentrations of BMAA in the rat's striatum 24h after the implantation of a microdialysis probe (day 1). BMAA perfusion produced a dose-response increase in the extracellular output of dopamine. Forty-eight hours after implantation of the probe (day 2), we have perfused MPP+ 1 mM to check the integrity of the dopaminergic terminals present around the cannula. Only the highest concentration of BMAA studied, 50mM, produced a clear decrease in the extracellular output of dopamine after MPP+ perfusion. However, this decrease was very similar, even smaller, to that obtained in a previous study carried out by us with MPP+ 1 mM, a dose much lower than that used for BMAA. Our model to study toxicity in the striatal dopaminergic terminal did not show that acute perfusion of BMAA at high doses produces a clear damage to the dopaminergic terminals.

• Authors: M Santiago, E R Matarredona, A Machado, J Cano
• Journal: Toxicology letters (Toxicol Lett) Vol. 167 Issue 1 Pg. 34-9 (Nov 01 2006) ISSN: 0378-4274 [Print] Netherlands
• PMID: 16979309 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Amino Acids, Diamino
• Cyanobacteria Toxins
• Excitatory Amino Acid Agonists
• 3,4-Dihydroxyphenylacetic Acid
• beta-N-methylamino-L-alanine
• Dopamine
• Homovanillic Acid

Topics

• 3,4-Dihydroxyphenylacetic Acid (metabolism)
• Amino Acids, Diamino (administration & dosage, toxicity)
• Animals
• Cell Death (drug effects)
• Cerebrovascular Circulation
• Chromatography, High Pressure Liquid
• Cyanobacteria Toxins
• Dopamine (metabolism)
• Electrochemistry
• Excitatory Amino Acid Agonists (administration & dosage, toxicity)
• Extracellular Space (drug effects, metabolism)
• Homovanillic Acid (metabolism)
• Microdialysis
• Neostriatum (blood supply, metabolism)
• Perfusion
• Rats
• Rats, Wistar