Abstract | PURPOSE:
Mucoepidermoid carcinoma is the most common primary malignancy of the salivary gland. Mucoepidermoid carcinoma translocated gene 1-mastermind-like gene family (MECT1-MAML2) gene fusion was identified from a recurring t(11;19)(q21;p13) translocation, which is often the sole cytogenetic alteration in this disease. This fusion transcript has been frequently detected in mucoepidermoid carcinoma and shown to be involved in the transformation of epithelial cells. However, its clinicopathologic significance remains unclear. EXPERIMENTAL DESIGN: Seventy-one cases of mucoepidermoid carcinoma and 51 cases of nonmucoepidermoid carcinoma salivary gland tumors (including 26 Warthin tumor cases) were retrospectively analyzed. RNA was extracted from archival materials: histologic paraffin specimens in all cases and cytologic specimens in 10 mucoepidermoid carcinoma cases. The MECT1-MAML2 fusion transcript was detected by a reverse transcription-PCR assay, which can be applied to both histologic and cytologic specimens. The presence of the fusion transcript was correlated with relevant clinicopathologic and survival data of the mucoepidermoid carcinoma patients. RESULTS: The MECT1-MAML2 fusion transcript was detected in 27 of the 71 (38%) mucoepidermoid carcinoma cases but not in any case of nonmucoepidermoid carcinoma tumors. The reverse transcription-PCR results showed no difference between histologic and cytologic specimens. Detection of the MECT1-MAML2 fusion transcript was associated with a less advanced clinical stage and a low-grade tumor histology. The presence of the transcript was associated with longer disease-free and overall survivals on univariate analysis and emerged as an independent prognostic factor for longer overall survival on multivariate analysis. CONCLUSIONS:
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Authors | Mitsukuni Okabe, Satoru Miyabe, Hitoshi Nagatsuka, Akihiro Terada, Nobuhiro Hanai, Motoo Yokoi, Kazuo Shimozato, Tadaaki Eimoto, Shigeo Nakamura, Noriyuki Nagai, Yasuhisa Hasegawa, Hiroshi Inagaki |
Journal | Clinical cancer research : an official journal of the American Association for Cancer Research
(Clin Cancer Res)
Vol. 12
Issue 13
Pg. 3902-7
(Jul 01 2006)
ISSN: 1078-0432 [Print] United States |
PMID | 16818685
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- MECT1-MAML2 fusion protein, human
- Oncogene Proteins, Fusion
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Topics |
- Adolescent
- Adult
- Aged
- Aged, 80 and over
- Carcinoma, Mucoepidermoid
(classification, genetics, pathology)
- Child
- Disease-Free Survival
- Female
- Gene Expression Profiling
- Gene Expression Regulation, Neoplastic
(genetics)
- Humans
- Male
- Middle Aged
- Multivariate Analysis
- Neoplasm Staging
- Oncogene Proteins, Fusion
(genetics)
- Retrospective Studies
- Reverse Transcriptase Polymerase Chain Reaction
(methods)
- Salivary Gland Neoplasms
(classification, genetics, pathology)
- Survival Rate
- Transcription, Genetic
(genetics)
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