Development of ELISA using recombinant antigens for specific detection of mouse parvovirus infection.

Abstract

Nucleotide sequences of mouse parvovirus (MPV) isolate, named MPV/UT, and mouse minute virus (MMV) were analyzed and used for expressing recombinant proteins in E. coli. ELISA tests using recombinant major capsid protein (rVP2) and recombinant major non-structural protein (rNS1) as antigens were developed and their performance in serologic detection of rodent parvovirus infection was assessed. MPV-rVP2 and MMV-rVP2 ELISAs reacted specifically with anti-MPV and anti-MMV mouse sera, respectively. MMV-rNS1 antigen had a wide reaction range with antisera to rodent parvoviruses including MPV, MMV, Kilham rat virus (KRV) and H-1 virus. All mice oronasally infected with MPV were seropositive at 4 weeks post-infection in screening by ELISAs using MPV-rVP2 and MMV-rNS1 antigens, but were negative by conventional ELISA using whole MMV antigen. A contact transmission experiment revealed that transmission of MPV occurred up to 4 weeks post-infection, and all cage mates were seropositive in screening with MPV-rVP2 and MMV-rNS1 ELISAs. These results indicate that MPV-rVP2 and MMV-rVP2 are specific ELISA antigens which distinguish between MPV and MVM infection, while MMV-rNS1 antigen can be used in generic ELISA for a variety of rodent parvoviruses. The higher sensitivity of MPV-rVP2 ELISA than conventional ELISA for detecting seroconversion to MPV in oronasally infected mice as well as in cage mates suggests the usefulness of MPV-rVP2 ELISA in quarantine and microbiological monitoring of MPV infection in laboratory mice.

• Authors: Satoshi Kunita, Miyuki Chaya, Kozue Hagiwara, Tomoko Ishida, Akira Takakura, Tatsuya Sugimoto, Hiroyoshi Iseki, Kumiko Fuke, Fumihiro Sugiyama, Ken-ichi Yagami
• Journal: Experimental animals (Exp Anim) Vol. 55 Issue 2 Pg. 117-24 (Apr 2006) ISSN: 1341-1357 [Print] Japan
• PMID: 16651694 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antigens, Viral
• Capsid Proteins
• Recombinant Proteins
• Viral Nonstructural Proteins

Topics

• Animals
• Antigens, Viral (analysis)
• Capsid Proteins (immunology)
• Enzyme-Linked Immunosorbent Assay (methods)
• Mice
• Minute Virus of Mice (immunology)
• Parvoviridae Infections (diagnosis, immunology, veterinary)
• Parvovirus (immunology)
• Recombinant Proteins (analysis)
• Rodent Diseases (diagnosis, immunology)
• Viral Nonstructural Proteins (immunology)