Cholesterol is required for efficient endoplasmic reticulum-to-Golgi transport of secretory membrane proteins.

Abstract

Although cholesterol is synthesized in the endoplasmic reticulum (ER), compared with other cellular membranes, ER membrane has low cholesterol (3-6%). Most of the molecular machinery that regulates cellular cholesterol homeostasis also resides in the ER. Little is known about how cholesterol itself affects the ER membrane. Here, we demonstrate that acute cholesterol depletion in ER membranes impairs ER-to-Golgi transport of secretory membrane proteins. Cholesterol depletion is achieved by a brief inhibition of cholesterol synthesis with statins in cells grown in cholesterol-depleted medium. We provide evidence that secretory membrane proteins vesicular stomatitis virus glycoprotein and scavenger receptor A failed to be efficiently transported from the ER upon cholesterol depletion. Fluorescence photobleaching recovery experiments indicated that cholesterol depletion by statins leads to a severe loss of lateral mobility on the ER membrane of these transmembrane proteins, but not loss of mobility of proteins in the ER lumen. This impaired lateral mobility is correlated with impaired ER-to-Golgi transport. These results provide evidence for the first time that cholesterol is required in the ER membrane to maintain mobility of membrane proteins and thus protein secretion.

• Authors: Andrew Ridsdale, Maxime Denis, Pierre-Yves Gougeon, Johnny K Ngsee, John F Presley, Xiaohui Zha
• Journal: Molecular biology of the cell (Mol Biol Cell) Vol. 17 Issue 4 Pg. 1593-605 (Apr 2006) ISSN: 1059-1524 [Print] United States
• PMID: 16452637 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adaptor Proteins, Signal Transducing
• G protein, vesicular stomatitis virus
• Hydroxymethylglutaryl-CoA Reductase Inhibitors
• Membrane Glycoproteins
• Membrane Proteins
• Viral Envelope Proteins
• Cholesterol
• Hydroxymethylglutaryl CoA Reductases
• Mevalonic Acid

Topics

• Adaptor Proteins, Signal Transducing (metabolism)
• Animals
• CHO Cells
• Cholesterol (deficiency)
• Cricetinae
• Cricetulus
• Endoplasmic Reticulum (drug effects, metabolism)
• Fluorescence Recovery After Photobleaching
• Golgi Apparatus (metabolism, ultrastructure)
• Hydroxymethylglutaryl CoA Reductases (drug effects)
• Hydroxymethylglutaryl-CoA Reductase Inhibitors (pharmacology)
• Membrane Glycoproteins (metabolism)
• Membrane Proteins (metabolism)
• Mevalonic Acid (pharmacology)
• Protein Transport
• Secretory Vesicles (drug effects, metabolism)
• Viral Envelope Proteins (metabolism)