A new specific gene expression in squamous cell carcinoma of the esophagus detected using representational difference analysis and cDNA microarray.

Abstract

OBJECTIVES:

To detect new specific gene expressions in squamous cell carcinoma of the esophagus.

METHODS:

Representational difference analysis of cDNA (cDNA RDA) was applied to a human esophageal cancer cell line (KYSE170) and a human esophageal epithelial cell line (HEEC-1).

RESULTS:

LAGE-1 was expressed specifically in KYSE170, but not in HEEC-1. It is also expressed in 27% of esophageal cancer cell lines (3/11) and 33% of esophageal cancer tissues (10/30), but not in other HEECs, normal esophageal epithelium, or other normal tissues except testis, ovary and kidney. The expression of LAGE-1 is strongly correlated with that of MAGE-A1 (p = 0.013, Fisher's exact probability test). Fibronectin, cytokeratin 6B, cytokeratin 19, cyclin D2 and Ten-m2 were detected as candidates for downregulated genes. Reduced expression profiles of them were also identified using cDNA microarrays. The expression of LAGE-1 was induced by 5'-aza-2'-deoxycytidine (5Aza-dC) and trichostatin A (TSA) in esophageal cancer cell lines, which did not express LAGE-1. In HEECs, 5Aza-dC induced LAGE-1 expression, but TSA did not.

CONCLUSIONS:

LAGE-1 expression was detected in esophageal cancer by cDNA RDA. LAGE-1 might have the potential to be a target antigen for anti-tumoral immunotherapy in esophageal cancers because of its tumor-specific expression similar to that of MAGE-A1.

• Authors: Takatsugu Kan, Seiji Yamasaki, Kan Kondo, Naoki Teratani, Atsushi Kawabe, Junichi Kaganoi, Stephen J Meltzer, Masayuki Imamura, Yutaka Shimada
• Journal: Oncology (Oncology) Vol. 70 Issue 1 Pg. 25-33 ( 2006) ISSN: 0030-2414 [Print] Switzerland
• PMID: 16446548 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
• Copyright: Copyright 2006 S. Karger AG, Basel.

Chemical References

• Antigens, Neoplasm
• Antigens, Surface
• Biomarkers, Tumor
• CTAG1B protein, human
• CTAG2 protein, human
• Membrane Proteins

Topics

• Antigens, Neoplasm (analysis)
• Antigens, Surface
• Biomarkers, Tumor (analysis)
• Carcinoma, Squamous Cell (chemistry, genetics)
• Cell Line, Tumor
• Cells, Cultured
• Epithelial Cells
• Esophageal Neoplasms (chemistry, genetics)
• Esophagus (chemistry, cytology)
• Gene Expression Regulation, Neoplastic
• Humans
• Membrane Proteins (analysis)
• Oligonucleotide Array Sequence Analysis
• Reverse Transcriptase Polymerase Chain Reaction
• Sequence Analysis, DNA