Cytogenetic effect of technical glyphosate on cultivated bovine peripheral lymphocytes.

Abstract

A technical herbicide containing isopropyl amine salt of glyphosate was tested for induction of chromosome aberrations (CA) and sister chromatid exchanges (SCE) in cultured bovine peripheral lymphocytes. Cultures were exposed to a glyphosate formulation at concentrations ranging from 28 to 1120 micromol/l without and with metabolic activation. No clastogenic effect of the herbicide was found. Its genotoxic effect was confirmed in the SCE assay after 24 h of incubation. A statistically significant elevation in SCE induction was observed in each of the donors after application of the product at doses ranging from 56 to 1120 micromol/l. The highest concentrations (560 and 1120 micromol/l) also caused reduction of mitotic and proliferation indices. In the 2 h-assay with metabolic activation a statistically significant frequency of SCE was observed only in cultures treated with the agent at a concentration of 140 micromol/l.

• Authors: Katarína Siviková, Ján Dianovskỳ
• Journal: International journal of hygiene and environmental health (Int J Hyg Environ Health) Vol. 209 Issue 1 Pg. 15-20 (Jan 2006) ISSN: 1438-4639 [Print] Germany
• PMID: 16373198 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• glyphosate, isopropyl amine salt
• glyphosate
• Glycine

Topics

• Animals
• Cattle
• Cell Proliferation
• Cells, Cultured
• Chromosome Aberrations (drug effects)
• Chromosomes, Mammalian (drug effects)
• Glycine (analogs & derivatives, toxicity)
• Lymphocytes (drug effects)
• Mitotic Index
• Sister Chromatid Exchange (drug effects)