Fas ligand reduces viability in primary melanoma short-term cell cultures more than in metastatic melanoma short-term cell cultures.

Abstract

BACKGROUND:

Apoptotic pathway aberrations are reported as important tumor progression factors in melanoma.

OBJECTIVE:

Effect of soluble Fas ligand (sFasL) and tumor necrosis factor-related apoptosis-inducing ligand (TRAIL) on short-term cultured melanoma cell viability from different stages of melanoma.

RESULTS:

Recombinant human FasL reduced viability after 18 h in a dose-dependent manner in 4 of 5 cell cultures from primary tumors and 1 of 9 cell cultures from metastatic melanoma (67.5 vs. 96.4%, p = 0.007). DNA fragmentation on flow cytometry confirmed apoptosis. Incubation with TRAIL had no effect on melanoma cell viability. Immunohistochemistry showed Fas in 3 of 4 primary and in 6 of 7 metastatic lesions, no FasL in primary lesions, and FasL in 5 of 7 metastatic lesions.

CONCLUSION:

Melanoma short-term cell cultures from primary tumors show decreased viability under FasL, but not TRAIL stimulation rather than short-term cell cultures derived from metastases.

• Authors: Günther F L Hofbauer, Naohito Hatta, Isabelle Daigle, Silvio Hemmi, Katharina Spanaus Schlapbach, Jörg Willers, Günter Burg, Hans-Uwe Simon, Reinhard Dummer
• Journal: Dermatology (Basel, Switzerland) (Dermatology) Vol. 211 Issue 4 Pg. 318-24 ( 2005) ISSN: 1018-8665 [Print] Switzerland
• PMID: 16286739 (Publication Type: Journal Article)
• Copyright: Copyright 2005 S. Karger AG, Basel.

Chemical References

• Antigens, Surface
• Apoptosis Regulatory Proteins
• FASLG protein, human
• Fas Ligand Protein
• Ligands
• Membrane Glycoproteins
• TNF-Related Apoptosis-Inducing Ligand
• TNFSF10 protein, human
• Tumor Necrosis Factor-alpha
• Tumor Necrosis Factors
• fas Receptor

Topics

• Antigens, Surface (analysis)
• Apoptosis (drug effects)
• Apoptosis Regulatory Proteins (pharmacology)
• Cell Survival (drug effects)
• DNA Fragmentation
• Dose-Response Relationship, Drug
• Fas Ligand Protein
• Flow Cytometry
• Humans
• Immunohistochemistry
• Jurkat Cells
• Ligands
• Lymphatic Metastasis (pathology)
• Melanoma (pathology, secondary)
• Membrane Glycoproteins (analysis, pharmacology)
• Neoplasm Staging
• TNF-Related Apoptosis-Inducing Ligand
• Time Factors
• Tumor Cells, Cultured
• Tumor Necrosis Factor-alpha (pharmacology)
• Tumor Necrosis Factors (analysis, pharmacology)
• fas Receptor (analysis, pharmacology)