The relationship of the histologic grade at the deep invasive front and the expression of Ki-67 antigen and p53 protein in oral squamous cell carcinoma.

Abstract

BACKGROUND:

Although many histopathologic characteristics of oral squamous cell carcinoma (O-SCC) have been identified as prognostic factors, accurate, and unequivocal factors have not been clearly identified. The purpose of this study was to evaluate a potential association between the histologic grade of malignancy at the deep invasive front and the expression of Ki-67 antigen and p53 protein in O-SCC.

METHODS:

The expression of Ki-67 antigen and p53 at the invasive tumor front area of O-SCC was examined by immunohistochemistry of archived tissue from 62 cases. The mean age of patients was 60.7 years (range: 37-89) and the male-female ratio was 1.6:1 (38 men, 24 women). There were 20, 17, 14, and 11 cases classified as stage I to stage IV, respectively. The correlation between the intensity of immunostaining for Ki-67 antigen and p53 and the histologic grade of malignancy at the deep invasive front (invasive front grade, IFG) was analyzed. The expression of Ki-67 antigen and p53 in normal oral epithelia (10 cases) was also investigated.

RESULTS:

The mean Ki-67 labeling index (LI) in the O-SCC samples was 32.8 +/- 12.0% (n = 62). The mean total score of IFG (IFG score) was 9.1 +/- 2.7 points (n = 62). There was a significant linear correlation between the IFG score and the Ki-67 antigen (gamma = 0.651, R2 = 0.596, P < 0.0001). Of 50 tumors examined, 27 (54.0%) exhibited p53-positive nuclear immunostaining. The staining patterns for Ki-67 antigen and p53 were similar. Both Ki-67-LI and p53-positive status were significantly correlated with the IFG scores.

CONCLUSION:

The findings of this study demonstrate that overexpression of Ki-67 antigen and p53 at the deep tumor invasive front of O-SCC is associated with histologic grade of malignancy.

• Authors: Hideo Kurokawa, Min Zhang, Shinobu Matsumoto, Yoshihiro Yamashita, Toshiko Tanaka, Taiki Tomoyose, Hirofumi Takano, Katsuyuki Funaki, Hiroshi Fukuyama, Tetsu Takahashi, Sumio Sakoda
• Journal: Journal of oral pathology & medicine : official publication of the International Association of Oral Pathologists and the American Academy of Oral Pathology (J Oral Pathol Med) Vol. 34 Issue 10 Pg. 602-7 (Nov 2005) ISSN: 0904-2512 [Print] Denmark
• PMID: 16202080 (Publication Type: Journal Article)

Chemical References

• Biomarkers, Tumor
• Ki-67 Antigen
• Tumor Suppressor Protein p53

Topics

• Adult
• Aged
• Aged, 80 and over
• Biomarkers, Tumor (analysis)
• Carcinoma, Squamous Cell (pathology)
• Female
• Humans
• Immunohistochemistry
• Ki-67 Antigen (analysis)
• Male
• Middle Aged
• Mouth Mucosa (pathology)
• Mouth Neoplasms (pathology)
• Neoplasm Invasiveness
• Neoplasm Staging
• Prognosis
• Tumor Suppressor Protein p53 (analysis)