Therapeutic immunization with dendritic cells loaded with heat-inactivated autologous HIV-1 in patients with chronic HIV-1 infection.

Abstract

Therapeutic immunization with autologous monocyte-derived dendritic cells (DCs) loaded with heat-inactivated autologous human immunodeficiency virus type 1 (HIV-1) in 12 patients with chronic HIV-1 infection who were receiving highly active antiretroviral therapy (HAART) was feasible, safe, and well tolerated. Virus was obtained during an initial interruption of HAART (hereafter, "stop 1") so that DCs could be pulsed. After immunization and a second interruption of HAART (hereafter, "stop 2"), set-point plasma viral load (PVL; 24 weeks after stop 2) decreased > or =0.5 log(10) copies/mL relative to baseline PVL in 4 of 12 patients. We observed a significant lengthening in mean doubling time of PVL rebound and significant decreases in the area under the curve and the mean peak of PVL rebound after stop 2, compared with those after stop 1. This response was associated with changes in HIV-1-specific CD4(+) lymphoproliferative and CD8(+) T cell responses. These changes were not observed in a group of nonimmunized control patients.

• Authors: Felipe García, Merylene Lejeune, Nuria Climent, Cristina Gil, José Alcamí, Vanessa Morente, Llucia Alós, Alba Ruiz, Javier Setoain, Emilio Fumero, Pedro Castro, Anna López, Anna Cruceta, Carlos Piera, Eric Florence, Arturo Pereira, Agnes Libois, Nuria González, Meritxell Guilá, Miguel Caballero, Francisco Lomeña, Joan Joseph, José M Miró, Tomás Pumarola, Montserrat Plana, José M Gatell, Teresa Gallart
• Journal: The Journal of infectious diseases (J Infect Dis) Vol. 191 Issue 10 Pg. 1680-5 (May 15 2005) ISSN: 0022-1899 [Print] United States
• PMID: 15838795 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• AIDS Vaccines
• Vaccines, Inactivated

Topics

• AIDS Vaccines (therapeutic use)
• Antiretroviral Therapy, Highly Active
• CD4-Positive T-Lymphocytes (physiology)
• CD8-Positive T-Lymphocytes (physiology)
• Dendritic Cells (immunology)
• HIV Infections (drug therapy, therapy)
• HIV-1 (immunology)
• Humans
• Vaccines, Inactivated (therapeutic use)
• Viral Load