Trypanosoma cruzi-infected individuals demonstrate varied antibody responses to a panel of trans-sialidase proteins encoded by SA85-1 genes.

Abstract

Chronic infection with Trypanosoma cruzi causes significant morbidity and mortality. The parasite expresses on its surface and sheds into the extracellular milieu a large superfamily of trans-sialidase proteins. Previous studies have demonstrated that during T. cruzi infection, the trans-sialidase superfamily stimulates an antibody response, but how individuals respond to different proteins of the trans-sialidase superfamily remain poorly defined. In this report, we present an analysis of the antibody response of chronically infected individuals and inbred strains of mice to a panel of 11 different trans-sialidase proteins encoded by surface antigen 85 kD (SA85-1) genes. These data indicate that: (1) 90% of the individuals tested generated antibodies to one or more trans-sialidase proteins; (2) the individuals develop different patterns of antibody responsiveness to the panel of trans-sialidase proteins; (3) three inbred strains of mice develop trans-sialidase antibody responses, but each strain develops a different pattern of antibody response to the panel of trans-sialidase proteins; (4) the differences in the pattern of antibody response by the mouse strains are independent of MHC differences; and (5) trans-sialidase proteins that do not stimulate an antibody response during T. cruzi infection can stimulate a response following immunization. Together these data indicate that during T. cruzi infection individuals develop a diverse trans-sialidase antibody response that appears to be affected by genetic and environmental factors.

• Authors: Malcolm S Duthie, Martin S Cetron, Wesley C Van Voorhis, Stuart J Kahn
• Journal: Acta tropica (Acta Trop) Vol. 93 Issue 3 Pg. 317-29 (Mar 2005) ISSN: 0001-706X [Print] Netherlands
• PMID: 15725381 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies, Protozoan
• Neuraminidase

Topics

• Animals
• Antibodies, Protozoan (drug effects)
• Antibody Formation (drug effects)
• Chagas Disease (immunology)
• Humans
• Mice
• Mice, Inbred BALB C
• Mice, Inbred C57BL
• Neuraminidase (genetics, pharmacology)
• Trypanosoma cruzi (enzymology, genetics)