Crystallization and preliminary X-ray diffraction analysis of suramin, a highly charged polysulfonated napthylurea, complexed with a myotoxic PLA2 from Bothrops asper venom.

Abstract

Suramin is a highly charged polysulfonated napthylurea that interferes in a number of physiologically relevant processes such as myotoxicity, blood coagulation and several kinds of cancers. This synthetic compound was complexed with a myotoxic Lys49 PLA(2) from Bothrops asper venom and crystallized by the hanging-drop vapor diffusion method at 18 degrees C. The crystals belong to the orthorhombic space group P2(1)2(1)2(1), with unit cell parameters a=49.05, b=63.84 and c=85.67 angstroms. Diffraction data was collected to 1.78 angstroms.

• Authors: Mário T Murakami, Lisandra M Gava, Sandro P Zela, Emerson Z Arruda, Paulo A Melo, José M Gutierrez, Raghuvir K Arni
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1703 Issue 1 Pg. 83-5 (Dec 01 2004) ISSN: 0006-3002 [Print] Netherlands
• PMID: 15588706 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Crotalid Venoms
• Suramin
• Urea
• Phospholipases A
• Group II Phospholipases A2
• Phospholipases A2
• Lysine

Topics

• Animals
• Bothrops
• Crotalid Venoms (chemistry, enzymology)
• Crystallography, X-Ray
• Group II Phospholipases A2
• Lysine (chemistry)
• Molecular Structure
• Phospholipases A (chemistry, isolation & purification, metabolism, toxicity)
• Phospholipases A2
• Suramin (analysis, chemistry, metabolism)
• Urea (analogs & derivatives, metabolism)
• X-Ray Diffraction