[Construction of the subtracted cDNA library in hypothalamus of the seasickness adaptive rats].

Abstract

OBJECTIVE:

To construct subtracted cDNA library in hypothalamus of the seasickness adaptive rats for providing theoretical basis for effective adaptive training against seasickness.

METHODS:

Suppression subtract hybridization technique was used, and forward and reverse hybridization was performed on the hypothalamus of seasickness adaptive rats and that of normal rats so that to construct subtracted cDNA library. Dot blot was used for differential screening the subtracted library.

RESULTS:

23 fragments of differentially expressed genes was obtained including 10 up-regulating and 13 down-regulating fragments.

CONCLUSION:

Many played role in adaptability formation to seasickness such as SAM, vasopressin, and heme oxygenase.

• Authors: Yanhong Ji, Junsheng Guo, Min Li, Faji Zhao
• Journal: Wei sheng yan jiu = Journal of hygiene research (Wei Sheng Yan Jiu) Vol. 33 Issue 4 Pg. 410-2 (Jul 2004) ISSN: 1000-8020 [Print] China
• PMID: 15461260 (Publication Type: English Abstract, Journal Article)

Chemical References

• DNA, Complementary

Topics

• Adaptation, Physiological (genetics)
• Animals
• Base Sequence
• DNA, Complementary (genetics)
• Gene Expression Regulation
• Gene Library
• Hypothalamus (metabolism)
• Male
• Motion Sickness (genetics, physiopathology)
• Nucleic Acid Hybridization
• Rats
• Rats, Sprague-Dawley