Host immunity, particularly T cell immunity (Th1/Th2 balance), plays an important role in clinicopathological features of malignant disease. However, the T cell immunity has not been fully investigated in patients with lymphoid
malignancies. Recent studies suggested the important role of dysregulation of the endogenous immune system in lymphomagenesis. The relationships between
cytokines/
chemokines and their receptors, are important in determining the selectivity of local immunity. To investigate differences in the endogenous immune system of
diffuse large B cell lymphoma (DLBL), we performed gene expression profiling using
cDNA microarrays of
cytokines/
chemokines and their receptors. We studied 5 cases each of primary central nervous system
lymphomas (PCNSL), extranodal and nodal
lymphomas. PCNSL exhibited diffuse down-regulated profiles, compared to normal peripheral blood lymphocytes. While extranodal and nodal
lymphomas also exhibited diffuse down-regulated profiles, some genes displayed up-regulated profiles. Hierarchical clustering analysis separated PCNSL and extranodal
lymphomas into distinct groups based on their gene expression profiles, as well as extranodal and nodal, but not PCNSL and nodal. PCNSL exhibited significantly lower expression of BLC/BCA-1 and CCR-3 (Th2 type), and higher expression of
IL-8 and
MIP-1beta (Th1 type) than extranodal
lymphomas. Immunohistochemistry and RT-PCR revealed frequent CCR-3 and BLC/BCA-1 expression in extranodal
lymphomas, compared with PCNSL. Our results provide new insights into the pathogenesis of each DLBL. A better understanding of the immune response in each DLBL could help in the design of novel therapeutic strategies based on
cytokines/
chemokines and their receptors.