Oral treatment with SRP299 (killed Mycobacterium vaccae) inhibits experimental periodontal disease in Wistar rats.

Abstract

OBJECTIVE:

Mycobacterium vaccae injected subcutaneously was previously shown to prevent and treat ligature-induced periodontal disease (PD) in Wistar rats (Breivik & Rook 2000, 2002). Since mycobacteria are readily taken up via Peyer's patches in the intestine, we have now tested the ability of oral SRP299 (killed M. vaccae) to prevent ligature-enhanced PD in Wistar rats, and to modulate the accompanying cytokine and corticosterone responses.

MATERIAL AND METHODS:

A single oral dose of SRP299 (1 mg) was given 14 days before the application of ligatures. PD was assessed when the ligatures had been in place for 56 days.

RESULTS:

Oral SRP299 reduced bone loss (p = 0.036, X-ray; p = 0.061, histometry) and fibre loss, both on the ligatured (p = 0.0047) and control (p = 0.005) sides, and also reduced the level of TNF-alpha (p = 0.0137) and corticosterone (p = 0.048) induced by intraperitoneal endotoxin lipopolysaccharide (LPS).

CONCLUSIONS:

SRP299 administered by the oral route diminishes ligature-induced bone and fibre loss in this model. This effect may be attributable to the known ability of SRP299 to evoke regulatory T cells, although the mechanism could not be investigated in this study. Treated rats also had less excitable hypothalamo-pituitary-adrenal (HPA) axes. HPA axis overactivity is a known risk factor in human PD. Trials of SRP299 in human PD are now justified.

• Authors: Torbjørn Breivik, Graham A W Rook
• Journal: Journal of clinical periodontology (J Clin Periodontol) Vol. 30 Issue 11 Pg. 931-6 (Nov 2003) ISSN: 0303-6979 [Print] United States
• PMID: 14761113 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Bacterial Vaccines
• SRP 299
• Tumor Necrosis Factor-alpha
• Vaccines, Inactivated
• Interleukin-10
• Corticosterone

Topics

• Administration, Oral
• Alveolar Bone Loss (immunology, prevention & control)
• Animals
• Bacterial Vaccines (administration & dosage, immunology)
• Corticosterone (blood)
• Interleukin-10 (blood)
• Male
• Maxilla
• Molar
• Mycobacterium (immunology)
• Mycobacterium Infections (prevention & control)
• Periodontal Diseases (immunology, prevention & control)
• Periodontal Ligament (immunology, pathology)
• Rats
• Rats, Wistar
• Serum (chemistry)
• Single-Blind Method
• Statistics, Nonparametric
• Tumor Necrosis Factor-alpha (analysis)
• Vaccines, Inactivated