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Specific oligonucleotide primers for identification of Cladophialophora carrionii, a causative agent of chromoblastomycosis.

Abstract
Cladophialophora carrionii is one of the relatively common causative agents of chromoblastomycosis. We have developed the specific oligonucleotide primer set based on the internal transcribed spacer regions of ribosomal DNA for the rapid identification of this pathogen. PCR with this primer set amplified a 362-bp amplicon from C. carrionii strains. From other relevant dematiaceous species, including medically important dematiaceous fungi, such as Fonsecaea pedrosoi, Phialophora verrucosa, and Exophiala dermatitidis, and eight species of medically important yeasts, such as Candida albicans and Cryptococcus neoformans var. neoformans, the primer set did not produce any amplicon. PCR with this primer set may be a useful tool for the identification of C. carrionii.
AuthorsParide Abliz, Kazutaka Fukushima, Kayoko Takizawa, Kazuko Nishimura
JournalJournal of clinical microbiology (J Clin Microbiol) Vol. 42 Issue 1 Pg. 404-7 (Jan 2004) ISSN: 0095-1137 [Print] United States
PMID14715791 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References
  • DNA Primers
Topics
  • Chromoblastomycosis (microbiology)
  • Cladosporium (classification, genetics, isolation & purification)
  • DNA Primers
  • Humans
  • Polymerase Chain Reaction (methods)

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