Abstract | BACKGROUND: METHODS: A rat model of peritoneal contamination and infection with human stool bacteria was used to simulate clinical trials that included increasing complexity. In trial 1, postoperative hypothermia (32 degrees C) was compared with normothermia (38 degrees C), without supportive treatment (10 rats per group). In trial 2, with a more severe infection, rats were given antibiotic prophylaxis. Using 20 rats per group, the authors compared postoperative hypothermia (32 degrees C), normothermia, and postoperative hypothermia (32 degrees C) with 20 mug/kg G-CSF prophylaxis given 12 h before surgery and 12 h and 36 h after surgery. The primary endpoint was death at 120 h. Secondary endpoints were systemic cytokine concentrations, leukocyte counts, and the phagocytic activity of granulocytes and monocytes. RESULTS: CONCLUSIONS: In this rat model of intraabdominal sepsis, postoperative hypothermia was deleterious. However, G-CSF treatment, initiated before contamination, reduced the mortality rate, increased the neutrophil count, and downgraded the systemic cytokine response.
|
Authors | Alexander Torossian, Sebastian Ruehlmann, Martin Middeke, Daniel I Sessler, Wilfried Lorenz, Hinnerk F Wulf, Artur Bauhofer |
Journal | Anesthesiology
(Anesthesiology)
Vol. 99
Issue 5
Pg. 1087-92
(Nov 2003)
ISSN: 0003-3022 [Print] United States |
PMID | 14576544
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
|
Chemical References |
- Chemokine CXCL2
- Cytokines
- Interleukin-6
- Monokines
- Tumor Necrosis Factor-alpha
- Granulocyte Colony-Stimulating Factor
|
Topics |
- Animals
- Antibiotic Prophylaxis
- Chemokine CXCL2
- Cytokines
(metabolism)
- Flow Cytometry
- Granulocyte Colony-Stimulating Factor
(therapeutic use)
- Granulocytes
(drug effects)
- Hypothermia, Induced
(adverse effects)
- Interleukin-6
(metabolism)
- Leukocyte Count
- Male
- Monocytes
(drug effects)
- Monokines
(metabolism)
- Neutrophils
- Phagocytosis
(drug effects)
- Rats
- Rats, Wistar
- Sepsis
(drug therapy, mortality, physiopathology)
- Stimulation, Chemical
- Survival
- Tumor Necrosis Factor-alpha
(metabolism)
|