Determination of cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid in the urine of a patient with cystathioninuria using column liquid chromatography-mass spectrometry.

Abstract

A method for the measurement of cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid in the urine of a patient with cystathioninuria has been developed, using column liquid chromatography-mass spectrometry. Cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid were determined by scanning the [M + H]+ ions of each compound. The recoveries were 80-92.4% for cystathionine and 80-100% for perhydro-1,4-thiazepine-3,5-dicarboxylic acid after ion-exchange treatment. The results agreed well with those obtained using an amino acid analyser. The concentrations found for cystathionine and perhydro-1,4-thiazepine-3,5-dicarboxylic acid were 1.289 +/- 0.099 mg/ml and 0.310 +/- 0.0067 mg/ml, respectively.

• Authors: K Sugahara, J Ohta, M Takemura, H Kodama
• Journal: Journal of chromatography (J Chromatogr) Vol. 579 Issue 2 Pg. 318-23 (Sep 02 1992) Netherlands
• PMID: 1429979 (Publication Type: Journal Article)

Chemical References

• Thiazepines
• Cystathionine
• perhydro-1,4-thiazepine-3,5-dicarboxylic acid

Topics

• Cystathionine (urine)
• Gas Chromatography-Mass Spectrometry (methods)
• Humans
• Thiazepines (urine)