Abstract | BACKGROUND: METHODS: Six bone marrow (BM) and 10 peripheral blood (PB) samples were obtained from 2 normal individuals, 3 patients with CML and 9 with ANLL. The CD34+ cell fraction was isolated using MY10 antibody, sheep anti-mouse immunomagnetic beads and the enzyme chymopapain. Indirect immunofluorescence and semisolid culture were employed to evaluate the percentage of CD34+ cells and that of clonogenic cells in each cell fraction. RESULTS: The frequency of CD34+ cells in the original unseparated populations was (mean +/- SE) 24.3 +/- 7.3%, and reached 85.0 +/- 2.7% in the isolated CD34-positive fractions; in the negative fractions it was only 2.7 +/- 1.7%. According to these results, the great majority of clonogenic cells was separated in the CD34-positive fractions and depleted in those CD34-negative. Moreover, chymopapain was shown to be non-toxic to the clonogenic cells. CONCLUSIONS: Positive immunoselection using My10 Ab, immunomagnetic beads and chymopapain is a method for isolating almost pure progenitors from the BM and PB of normal individuals and patients with myeloid leukemias.
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Authors | F Silvestri, S Banavali, M Yin, V Gopal, C Savignano, M Baccarani, H D Preisler |
Journal | Haematologica
(Haematologica)
1992 Jul-Aug
Vol. 77
Issue 4
Pg. 307-10
ISSN: 0390-6078 [Print] Italy |
PMID | 1385276
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antigens, CD
- Antigens, CD34
- Antigens, Neoplasm
- Biomarkers, Tumor
- Chymopapain
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Topics |
- Antigens, CD
- Antigens, CD34
- Antigens, Neoplasm
- Biomarkers, Tumor
- Bone Marrow
(pathology)
- Cell Separation
(methods)
- Chymopapain
- Fluorescent Antibody Technique
- Hematopoietic Stem Cells
(immunology)
- Humans
- Immunosorbent Techniques
- Leukemia, Myelogenous, Chronic, BCR-ABL Positive
(pathology)
- Leukemia, Myeloid, Acute
(pathology)
- Magnetics
- Microspheres
- Neoplastic Stem Cells
(immunology)
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