ELG1, a yeast gene required for genome stability, forms a complex related to replication factor C.

Abstract	Many overlapping surveillance and repair mechanisms operate in eukaryotic cells to ensure the stability of the genome. We have screened to isolate yeast mutants exhibiting increased levels of recombination between repeated sequences. Here we characterize one of these mutants, elg1. Strains lacking Elg1p exhibit elevated levels of recombination between homologous and nonhomologous chromosomes, as well as between sister chromatids and direct repeats. These strains also exhibit increased levels of chromosome loss. The Elg1 protein shares sequence homology with the large subunit of the clamp loader replication factor C (RFC) and with the product of two additional genes involved in checkpoint functions and genome maintenance: RAD24 and CTF18. Elg1p forms a complex with the Rfc2-5 subunits of RFC that is distinct from the previously described RFC-like complexes containing Rad24 and Ctf18. Genetic data indicate that the Elg1, Ctf18, and Rad24 RFC-like complexes work in three separate pathways important for maintaining the integrity of the genome and for coping with various genomic stresses.
Authors	Shay Ben-Aroya, Amnon Koren, Batia Liefshitz, Rivka Steinlauf, Martin Kupiec
Journal	Proceedings of the National Academy of Sciences of the United States of America (Proc Natl Acad Sci U S A) Vol. 100 Issue 17 Pg. 9906-11 (Aug 19 2003) ISSN: 0027-8424 [Print] United States
PMID	12909721 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	CTF4 protein, S cerevisiae Carrier Proteins DNA-Binding Proteins Elg1 protein, S cerevisiae Proteins Saccharomyces cerevisiae Proteins Replication Protein C
Topics	Amino Acid Sequence Carrier Proteins (genetics) Crossing Over, Genetic DNA Replication (genetics) DNA-Binding Proteins (genetics) Gene Conversion Gene Deletion Genes, Fungal Genome, Fungal Molecular Sequence Data Mutation Proteins Recombination, Genetic Replication Protein C Saccharomyces cerevisiae (genetics, metabolism) Saccharomyces cerevisiae Proteins (genetics) Sequence Homology, Amino Acid Sister Chromatid Exchange

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