Abstract | PURPOSE: METHODS: Viruses used for the experiment were clinically isolated AdV type 3(AdV 3), AdV type 4(AdV 4), type 8(ADV 8), AdV type 19(AdV 19), and type 37(AdV 37). We examined three antiviral agents, i.e., NMSO 3, cidofovir( HPMPC), and zalcitabine(ddC). 50% effective concentration(EC50), 50% cytotoxic concentration(CC50), and selectivity index(SI) of compounds were determined for AdV infection in HEp-2 cells using 3-(4,5-dimetyl-thiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) methods. We also evaluated the anti-AdV activity of NMSO 3 when it was added during the stage of virus adsorption. RESULTS:
NMSO 3, HPMPC, and ddC showed an inhibitory effect against all five AdV clinical strains. The EC50 values of NMSO3 were lower than those of HPMPC and ddC. NMSO 3 exhibited minimal cytotoxicity. NMSO 3 inhibited AdV infection only when it was added during the stage of virus adsorption. CONCLUSIONS:
NMSO 3 inhibited the replication of all clinical AdV serotypes tested. NMSO 3 was the most potent and selective anti-AdV compound. The mechanism of anti-AdV activity by NMSO 3 was inhibition of viral adsorption to cells.
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Authors | Hisatoshi Kaneko, Shuichi Mori, Shiro Shigeta, Shigeaki Ohno, Koki Aoki |
Journal | Nippon Ganka Gakkai zasshi
(Nippon Ganka Gakkai Zasshi)
Vol. 107
Issue 4
Pg. 196-201
(Apr 2003)
ISSN: 0029-0203 [Print] Japan |
PMID | 12755063
(Publication Type: Comparative Study, Journal Article)
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Chemical References |
- (2,2-bis(docosyloxymethyl)propyl-5-acetamido-3,5-dideoxy-4,7,8,9-tetra-O-(sodium oxysulfonyl)glycero-alpha-galacto-2-nonulopyranosid)onate
- Antiviral Agents
- Lipids
- Organophosphonates
- Organophosphorus Compounds
- Zalcitabine
- Cytosine
- N-Acetylneuraminic Acid
- Cidofovir
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Topics |
- Adenoviridae
(drug effects)
- Antiviral Agents
(pharmacology)
- Cells, Cultured
- Cidofovir
- Cytosine
(analogs & derivatives, pharmacology)
- Humans
- Lipids
(pharmacology)
- N-Acetylneuraminic Acid
(analogs & derivatives, pharmacology)
- Organophosphonates
- Organophosphorus Compounds
(pharmacology)
- Zalcitabine
(pharmacology)
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