HNK-1-Reactive oligosaccharide, sulfate-O-3GlcAbeta1-4Xylbeta1-MU, synthesized by cultured human colorectal cancer cells.

Abstract	Human colorectal cancer cells were incubated with medium containing 4-methylumbelliferyl-beta-D-xyloside (Xyl-MU). The cells synthesized Xyl-MU-derivatives which were detected in the culture medium by gel-filtration high-performance liquid chromatography. These included a Xyl-MU-induced glycosaminoglycan and its biosynthetic intermediates, Galbeta1-4Xylbeta1-MU and Galbeta1-3Galbeta1-4Xylbeta1-MU, and other Xyl-MU-induced oligosaccharides, not related to Xyl-MU-induced glycosaminoglycan, were also synthesized. One of these oligosaccharides, sulfate-O-3GlcAbeta1-4Xylbeta1-MU, reacted with HNK-1, a mouse monoclonal antibody raised against human natural killer cells. Human neural cells and skin fibroblasts have also been reported to synthesize HNK-1-reactive sugar chains. Since HNK-1-reactive sugar chains are known to be involved in cell adhesion in the nervous system, the present results suggest that epithelium-derived colorectal cancer cells might also be able to utilize them in cell adhesion.
Authors	Shigeru Shibata, Keiichi Takagaki, Keinosuke Ishido, Mitsuru Konn, Mutsuo Sasaki, Masahiko Endo
Journal	The Tohoku journal of experimental medicine (Tohoku J Exp Med) Vol. 199 Issue 1 Pg. 13-23 (Jan 2003) ISSN: 0040-8727 [Print] Japan
PMID	12688556 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	3-glucuronosyl-1-4-(xylosyl-1-)-4-methylumbelliferone sulfate Antibodies, Monoclonal CD57 Antigens Culture Media Disaccharides Glycosaminoglycans Oligosaccharides Hymecromone methylumbelliferyl-beta-D-xyloside Glucuronidase
Topics	Animals Antibodies, Monoclonal (drug effects) CD57 Antigens (drug effects) Carbohydrate Sequence Chromatography, Gel Chromatography, High Pressure Liquid Colorectal Neoplasms (metabolism) Culture Media Disaccharides (chemistry) Glucuronidase (chemistry) Glycosaminoglycans (chemistry) Humans Hydrolysis Hymecromone (analogs & derivatives, chemistry, pharmacology) Mice Molecular Sequence Data Oligosaccharides (pharmacology) Tumor Cells, Cultured

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